Impedimetric cardiac biomarker determination in serum mediated by epoxy and hydroxyl of reduced graphene oxide on gold array microelectrodes.

Autor: Taniselass S; Faculty of Electronic Engineering Technology, Universiti Malaysia Perlis, Pauh Putra Campus, 02600, Arau, Perlis, Malaysia.; Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, 01000, Kangar, Perlis, Malaysia., Arshad MKM; Faculty of Electronic Engineering Technology, Universiti Malaysia Perlis, Pauh Putra Campus, 02600, Arau, Perlis, Malaysia. mohd.khairuddin@unimap.edu.my.; Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, 01000, Kangar, Perlis, Malaysia. mohd.khairuddin@unimap.edu.my., Gopinath SCB; Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, 01000, Kangar, Perlis, Malaysia.; Faculty of Chemical Engineering Technology, Universiti Malaysia Perlis, 02600, Arau, Perlis, Malaysia., Fathil MFM; Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, 01000, Kangar, Perlis, Malaysia., Ibau C; Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, 01000, Kangar, Perlis, Malaysia., Anbu P; Department of Biological Engineering, College of Engineering, Inha University, Incheon, 402-751, Republic of Korea.
Jazyk: angličtina
Zdroj: Mikrochimica acta [Mikrochim Acta] 2021 Jul 15; Vol. 188 (8), pp. 257. Date of Electronic Publication: 2021 Jul 15.
DOI: 10.1007/s00604-021-04922-x
Abstrakt: A label-free chemical bonding strategy mediated by reduced graphene oxide (rGO) basal plane functional groups has been developed for cardiac Troponin I (cTnI) detection. Four different chemical strategies on respective electrode sensing surface were precedingly examined using electrochemical impedance spectroscopy. The impedimetric assessment was carried out by sweeping frequency at the range 0.1-500 kHz perturbated at a small amplitude of AC voltage (25 mV). The chemical strategy-4 denoted as S-4 shows a significant analytical performance on cTnI detection in spiked buffer and human serum, whereby the pre-mixture of rGO and (3-Aminopropyl)triethoxysilane (APTES) creates a large number of amine sites (-NH 2 ), which significantly enhanced the antibody immobilization without excessive functionalization. The as-fabricated immunosensor exhibited an ultra-low limit of detection of 6.3 ag mL -1 and the lowest antigen concentration measured was at 10 ag mL -1 . The immunosensor showed a linear and wide range of cTnI detection (10 ag mL -1 -100 ng mL -1 ) in human serum with a regression coefficient of 0.9716, rapid detection (5 min of binding time), and stable and highly reproducible bioelectrode response with RSD < 5%. Hence, the demonstrated S-4 strategy is highly recommended for other downstream biosensors applications.
(© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)
Databáze: MEDLINE
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