Longitudinal variation in SARS-CoV-2 antibody levels and emergence of viral variants: implications for the ability of serological assays to predict immunity.

Autor:	Muecksch F; Laboratory of Retrovirology, The Rockefeller University, 1230 York Avenue, New York NY 10065., Wise H; Royal Infirmary of Edinburgh, NHS Lothian, 51 Little France Crescent, Edinburgh EH16 4SA., Templeton K; Royal Infirmary of Edinburgh, NHS Lothian, 51 Little France Crescent, Edinburgh EH16 4SA., Batchelor B; Western General Hospital, Crewe Road South, Edinburgh, EH4 2XU., Squires M; Royal Infirmary of Edinburgh, NHS Lothian, 51 Little France Crescent, Edinburgh EH16 4SA., McCance K; Royal Infirmary of Edinburgh, NHS Lothian, 51 Little France Crescent, Edinburgh EH16 4SA., Jarvis L; SNBTS Microbiology Reference Laboratory, The Jack Copland Centre, 52 Research Avenue North, Heriot Watt Research Park, Edinburgh, EH14 4AP., Malloy K; SNBTS Microbiology Reference Laboratory, The Jack Copland Centre, 52 Research Avenue North, Heriot Watt Research Park, Edinburgh, EH14 4AP., Furrie E; Ninewells Hospital and Medical School, NHS Tayside, Dundee, DD1 9SY., Richardson C; University Hospital Monklands, NHS Lanarkshire, Airdrie, ML6 0JS., MacGuire J; University Hospital Monklands, NHS Lanarkshire, Airdrie, ML6 0JS., Godber I; Queen Elizabeth University Hospital, Govan Road, Glasgow, G51 4TF., Burns A; Queen Elizabeth University Hospital, Govan Road, Glasgow, G51 4TF., Mavin S; Scottish Microbiology Reference Laboratory, NHS Highland, Inverness, IV2 3UJ., Zhang F; Laboratory of Retrovirology, The Rockefeller University, 1230 York Avenue, New York NY 10065., Schmidt F; Laboratory of Retrovirology, The Rockefeller University, 1230 York Avenue, New York NY 10065., Bieniasz P; Laboratory of Retrovirology, The Rockefeller University, 1230 York Avenue, New York NY 10065.; Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York NY 10065., Jenks S; Royal Infirmary of Edinburgh, NHS Lothian, 51 Little France Crescent, Edinburgh EH16 4SA., Hatziioannou T; Laboratory of Retrovirology, The Rockefeller University, 1230 York Avenue, New York NY 10065.
Jazyk:	angličtina
Zdroj:	MedRxiv : the preprint server for health sciences [medRxiv] 2021 Jul 07. Date of Electronic Publication: 2021 Jul 07.
DOI:	10.1101/2021.07.02.21259939
Abstrakt:	Background: Serological assays are being deployed to monitor antibody responses in SARS-CoV-2 convalescents and vaccine recipients. There is a need to determine whether such assays can predict immunity, as antibody levels wane and viral variants emerge. Methods: We measured antibodies in a cohort of SARS-CoV-2 infected patients using several high-throughput serological tests and functional neutralization assays. The effects of time and spike protein sequence variation on the performance and predictive value of the various assays was assessed. Findings: Neutralizing antibody titers decreased over the first few months post-infection but stabilized thereafter, at about 30% of the level observed shortly after infection. Serological assays commonly used to measure antibodies against SARS-CoV-2 displayed a range of sensitivities that declined to varying extents over time. Quantitative measurements generated by serological assays based on the spike protein were better at predicting neutralizing antibody titers than assays based on nucleocapsid, but performance was variable and manufacturer positivity thresholds were not able to predict the presence or absence of detectable neutralizing activity. Even though there was some deterioration in correlation between serological measurements and functional neutralization activity, some assays maintained an ability to predict neutralizing titers, even against variants of concern. Interpretation: The ability of high throughput serological assays to predict neutralizing antibody titers is likely crucial for evaluation of immunity at the population scale. These data will facilitate the selection of the most suitable assays as surrogates of functional neutralizing activity and suggest that such measurements may have utility in clinical practice.
Databáze:	MEDLINE
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