Towards engineering heart tissues from bioprinted cardiac spheroids.

Autor:	Polonchuk L; F Hoffmann-La Roche AG Research and Development Division, Pharmaceutical Sciences, Roche Innovation Center Basel, Grenzacherstrasse 124, Basel, Basel-Stadt CH-4070, Switzerland., Surija L; The University of Sydney Faculty of Medicine and Health, Kolling Building, Kolling Institute, St Leonards, Sydney, NSW 2065, Australia., Lee MH; The University of Sydney Faculty of Medicine and Health, Kolling Building, Kolling Institute, St Leonards, Sydney, NSW 2065, Australia., Sharma P; The University of Sydney Faculty of Medicine and Health, Kolling Building, Kolling Institute, St Leonards, Sydney, NSW 2065, Australia.; The University of Newcastle Faculty of Health and Medicine, University Drive, Callaghan, NSW 2308, Australia.; University of Technology Sydney Faculty of Engineering and IT, Building 11, Level 10, Room 115, Ultimo, Sydney, NSW 2007, Australia., Liu Chung Ming C; University of Technology Sydney Faculty of Engineering and IT, Building 11, Level 10, Room 115, Ultimo, Sydney, NSW 2007, Australia., Richter F; The University of Sydney Faculty of Medicine and Health, Kolling Building, Kolling Institute, St Leonards, Sydney, NSW 2065, Australia., Ben-Sefer E; University of Technology Sydney Faculty of Engineering and IT, Building 11, Level 10, Room 115, Ultimo, Sydney, NSW 2007, Australia., Rad MA; University of Technology Sydney Faculty of Engineering and IT, Building 11, Level 10, Room 115, Ultimo, Sydney, NSW 2007, Australia., Mahmodi Sheikh Sarmast H; University of Technology Sydney Faculty of Engineering and IT, Building 11, Level 10, Room 115, Ultimo, Sydney, NSW 2007, Australia., Shamery WA; University of Technology Sydney Faculty of Engineering and IT, Building 11, Level 10, Room 115, Ultimo, Sydney, NSW 2007, Australia., Tran HA; School of Biomedical Engineering, UNSW Sydney, Sydney, NSW 2052, Australia., Vettori L; University of Technology Sydney Faculty of Engineering and IT, Building 11, Level 10, Room 115, Ultimo, Sydney, NSW 2007, Australia., Haeusermann F; F Hoffmann-La Roche AG Research and Development Division, Pharmaceutical Sciences, Roche Innovation Center Basel, Grenzacherstrasse 124, Basel, Basel-Stadt CH-4070, Switzerland., Filipe EC; Garvan Institute of Medical Research, 384 Victoria St, Darlinghurst, NSW 2010, Australia.; St Vincent Clinical School, Faculty of Medicine, UNSW Sydney, Sydney, NSW 2052, Australia., Rnjak-Kovacina J; School of Biomedical Engineering, UNSW Sydney, Sydney, NSW 2052, Australia., Cox T; Garvan Institute of Medical Research, 384 Victoria St, Darlinghurst, NSW 2010, Australia.; St Vincent Clinical School, Faculty of Medicine, UNSW Sydney, Sydney, NSW 2052, Australia., Tipper J; University of Technology Sydney Faculty of Engineering and IT, Building 11, Level 10, Room 115, Ultimo, Sydney, NSW 2007, Australia., Kabakova I; University of Technology Sydney Faculty of Engineering and IT, Building 11, Level 10, Room 115, Ultimo, Sydney, NSW 2007, Australia., Gentile C; The University of Sydney Faculty of Medicine and Health, Kolling Building, Kolling Institute, St Leonards, Sydney, NSW 2065, Australia.; University of Technology Sydney Faculty of Engineering and IT, Building 11, Level 10, Room 115, Ultimo, Sydney, NSW 2007, Australia.
Jazyk:	angličtina
Zdroj:	Biofabrication [Biofabrication] 2021 Aug 13; Vol. 13 (4). Date of Electronic Publication: 2021 Aug 13.
DOI:	10.1088/1758-5090/ac14ca
Abstrakt:	Current in vivo and in vitro models fail to accurately recapitulate the human heart microenvironment for biomedical applications. This study explores the use of cardiac spheroids (CSs) to biofabricate advanced in vitro models of the human heart. CSs were created from human cardiac myocytes, fibroblasts and endothelial cells (ECs), mixed within optimal alginate/gelatin hydrogels and then bioprinted on a microelectrode plate for drug testing. Bioprinted CSs maintained their structure and viability for at least 30 d after printing. Vascular endothelial growth factor (VEGF) promoted EC branching from CSs within hydrogels. Alginate/gelatin-based hydrogels enabled spheroids fusion, which was further facilitated by addition of VEGF. Bioprinted CSs contracted spontaneously and under stimulation, allowing to record contractile and electrical signals on the microelectrode plates for industrial applications. Taken together, our findings indicate that bioprinted CSs can be used to biofabricate human heart tissues for long term in vitro testing. This has the potential to be used to study biochemical, physiological and pharmacological features of human heart tissue. (Creative Commons Attribution license.)
Databáze:	MEDLINE
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