Isolation of intact high-molecular-weight DNA by using guanidine isothiocyanate.

Autor: Lippke JA; BioTechnica Diagnostics, Cambridge, Massachusetts 02138., Strzempko MN, Raia FF, Simon SL, French CK
Jazyk: angličtina
Zdroj: Applied and environmental microbiology [Appl Environ Microbiol] 1987 Oct; Vol. 53 (10), pp. 2588-9.
DOI: 10.1128/aem.53.10.2588-2589.1987
Abstrakt: A method for obtaining high-molecular-weight chromosomal DNA from Bacteroides intermedius and Bacteroides gingivalis is described. This technique is a modification of the guanidine isothiocyanate isolation procedure for RNA and should be useful for isolating intact DNA from organisms with high endogenous nuclease activity.
Databáze: MEDLINE