Development of a free radical scavenging bacterial consortium to mitigate oxidative stress in cnidarians.

Autor: Dungan AM; School of Biosciences, The University of Melbourne, Melbourne, Vic., Australia., Bulach D; Melbourne Bioinformatics, The University of Melbourne, Melbourne, Vic., Australia., Lin H; School of Earth Sciences, The University of Melbourne, Melbourne, Vic., Australia., van Oppen MJH; School of Biosciences, The University of Melbourne, Melbourne, Vic., Australia.; Australian Institute of Marine Science, Townsville, Qld, Australia., Blackall LL; School of Biosciences, The University of Melbourne, Melbourne, Vic., Australia.
Jazyk: angličtina
Zdroj: Microbial biotechnology [Microb Biotechnol] 2021 Sep; Vol. 14 (5), pp. 2025-2040. Date of Electronic Publication: 2021 Jul 14.
DOI: 10.1111/1751-7915.13877
Abstrakt: Corals are colonized by symbiotic microorganisms that profoundly influence the animal's health. One noted symbiont is a single-celled alga (in the dinoflagellate family Symbiodiniaceae), which provides the coral with most of its fixed carbon. Thermal stress increases the production of reactive oxygen species (ROS) by Symbiodiniaceae during photosynthesis. ROS can both damage the algal symbiont's photosynthetic machinery and inhibit its repair, causing a positive feedback loop for the toxic accumulation of ROS. If not scavenged by the antioxidant network, excess ROS may trigger a signaling cascade ending with the coral host and algal symbiont disassociating in a process known as bleaching. We use Exaiptasia diaphana as a model for corals and constructed a consortium comprised of E. diaphana-associated bacteria capable of neutralizing ROS. We identified six strains with high free radical scavenging (FRS) ability belonging to the families Alteromonadaceae, Rhodobacteraceae, Flavobacteriaceae and Micrococcaceae. In parallel, we established a consortium of low FRS isolates consisting of genetically related strains. Bacterial whole genome sequences were used to identify key pathways that are known to influence ROS.
(© 2021 The Authors. Microbial Biotechnology published by Society for Applied Microbiology and John Wiley & Sons Ltd.)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje