Discrimination of deletion to point cytokine mutants based on an array of cross-reactive receptors mimicking protein recognition by heparan sulfate.

Autor: Genua M; Université Grenoble Alpes, CEA, CNRS, IRIG-SyMMES, 38000, Grenoble, France., Garçon LA; Université Grenoble Alpes, CEA, CNRS, IRIG-SyMMES, 38000, Grenoble, France., Sergeeva YN; Université Grenoble Alpes, CEA, CNRS, IRIG-SyMMES, 38000, Grenoble, France., Saesen E; Institut de Biologie Structurale, UMR 5075, Université Grenoble Alpes, CNRS, CEA, 38000, Grenoble, France., Musnier B; Université Grenoble Alpes, CEA, CNRS, IRIG-SyMMES, 38000, Grenoble, France., Buhot A; Université Grenoble Alpes, CEA, CNRS, IRIG-SyMMES, 38000, Grenoble, France., Billon M; Université Grenoble Alpes, CEA, CNRS, IRIG-SyMMES, 38000, Grenoble, France., Gout E; Institut de Biologie Structurale, UMR 5075, Université Grenoble Alpes, CNRS, CEA, 38000, Grenoble, France., Sadir R; Institut de Biologie Structurale, UMR 5075, Université Grenoble Alpes, CNRS, CEA, 38000, Grenoble, France., Lortat-Jacob H; Institut de Biologie Structurale, UMR 5075, Université Grenoble Alpes, CNRS, CEA, 38000, Grenoble, France., Le Narvor C; Université Paris-Saclay, CNRS, Institut de chimie moléculaire et des matériaux d'Orsay, 91405, Orsay, France., Bonnaffé D; Université Paris-Saclay, CNRS, Institut de chimie moléculaire et des matériaux d'Orsay, 91405, Orsay, France., Livache T; Université Grenoble Alpes, CEA, CNRS, IRIG-SyMMES, 38000, Grenoble, France., Hou Y; Université Grenoble Alpes, CEA, CNRS, IRIG-SyMMES, 38000, Grenoble, France. Yanxia.hou-broutin@cea.fr.
Jazyk: angličtina
Zdroj: Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2022 Jan; Vol. 414 (1), pp. 551-559. Date of Electronic Publication: 2021 Jul 13.
DOI: 10.1007/s00216-021-03516-z
Abstrakt: Differential sensing of proteins based on cross-reactive arrays and pattern recognition is a promising technique for the detection and identification of proteins. In this study, a rational biomimetic strategy has been used to prepare sensing materials capable of discriminating structurally similar proteins, such as deletion and point mutants of a cytokine, by mimicking the biological properties of heparan sulfate (HS). Using the self-assembly of two disaccharides, lactose and sulfated lactose at various ratios on the surface of a chip, an array of combinatorial cross-reactive receptors has been prepared. Coupling with surface plasmon resonance imaging (SPRi), the obtained cross-reactive array is very efficient for protein sensing. It is able to detect HS binding proteins (HSbps) such as IFNγ at nanomolar concentrations. Moreover, such a system is capable of discriminating between IFNγ and its mutants with good selectivity.
(© 2021. Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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