A Trimethylguanosine Synthase1-like (TGS1) homologue is implicated in vernalisation and flowering time control.

Autor: Taylor CM; UWA School of Agriculture and Environment, The University of Western Australia, Perth, WA, 6009, Australia.; The UWA Institute of Agriculture, The University of Western Australia, Perth, WA, 6009, Australia., Garg G; Agriculture and Food, Commonwealth Scientific and Industrial Research Organisation, Floreat, WA, 6014, Australia., Berger JD; Agriculture and Food, Commonwealth Scientific and Industrial Research Organisation, Floreat, WA, 6014, Australia., Ribalta FM; UWA School of Agriculture and Environment, The University of Western Australia, Perth, WA, 6009, Australia., Croser JS; UWA School of Agriculture and Environment, The University of Western Australia, Perth, WA, 6009, Australia.; The UWA Institute of Agriculture, The University of Western Australia, Perth, WA, 6009, Australia., Singh KB; The UWA Institute of Agriculture, The University of Western Australia, Perth, WA, 6009, Australia.; Agriculture and Food, Commonwealth Scientific and Industrial Research Organisation, Floreat, WA, 6014, Australia.; Centre for Crop and Disease Management, Curtin University, Bentley, WA, 6102, Australia., Cowling WA; UWA School of Agriculture and Environment, The University of Western Australia, Perth, WA, 6009, Australia. wallace.cowling@uwa.edu.au.; The UWA Institute of Agriculture, The University of Western Australia, Perth, WA, 6009, Australia. wallace.cowling@uwa.edu.au., Kamphuis LG; The UWA Institute of Agriculture, The University of Western Australia, Perth, WA, 6009, Australia.; Agriculture and Food, Commonwealth Scientific and Industrial Research Organisation, Floreat, WA, 6014, Australia.; Centre for Crop and Disease Management, Curtin University, Bentley, WA, 6102, Australia., Nelson MN; The UWA Institute of Agriculture, The University of Western Australia, Perth, WA, 6009, Australia.; Agriculture and Food, Commonwealth Scientific and Industrial Research Organisation, Floreat, WA, 6014, Australia.
Jazyk: angličtina
Zdroj: TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik [Theor Appl Genet] 2021 Oct; Vol. 134 (10), pp. 3411-3426. Date of Electronic Publication: 2021 Jul 13.
DOI: 10.1007/s00122-021-03910-2
Abstrakt: Key Message: A plant-specific Trimethylguanosine Synthase1-like homologue was identified as a candidate gene for the efl mutation in narrow-leafed lupin, which alters phenology by reducing vernalisation requirement. The vernalisation pathway is a key component of flowering time control in plants from temperate regions but is not well understood in the legume family. Here we examined vernalisation control in the temperate grain legume species, narrow-leafed lupin (Lupinus angustifolius L.), and discovered a candidate gene for an ethylene imine mutation (efl). The efl mutation changes phenology from late to mid-season flowering and additionally causes transformation from obligate to facultative vernalisation requirement. The efl locus was mapped to pseudochromosome NLL-10 in a recombinant inbred line (RIL) mapping population developed by accelerated single seed descent. Candidate genes were identified in the reference genome, and a diverse panel of narrow-leafed lupins was screened to validate mutations specific to accessions with efl. A non-synonymous SNP mutation within an S-adenosyl-L-methionine-dependent methyltransferase protein domain of a Trimethylguanosine Synthase1-like (TGS1) orthologue was identified as the candidate mutation giving rise to efl. This mutation caused substitution of an amino acid within an established motif at a position that is otherwise highly conserved in several plant families and was perfectly correlated with the efl phenotype in F 2 and F 6 genetic population and a panel of diverse accessions, including the original efl mutant. Expression of the TGS1 homologue did not differ between wild-type and efl genotypes, supporting altered functional activity of the gene product. This is the first time a TGS1 orthologue has been associated with vernalisation response and flowering time control in any plant species.
(© 2021. The Author(s).)
Databáze: MEDLINE
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