Isolation of a novel insect-specific flavivirus with immunomodulatory effects in vertebrate systems.

Autor: Auguste AJ; Department of Entomology, College of Agriculture and Life Sciences, Fralin Life Science Institute, Virginia Polytechnic Institute and State University, Blacksburg, VA, 24061, USA; Center for Emerging, Zoonotic, and Arthropod-borne Pathogens, Virginia Polytechnic Institute and State University, Blacksburg, VA, 24061, USA. Electronic address: jauguste@vt.edu., Langsjoen RM; Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA., Porier DL; Department of Entomology, College of Agriculture and Life Sciences, Fralin Life Science Institute, Virginia Polytechnic Institute and State University, Blacksburg, VA, 24061, USA., Erasmus JH; Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA., Bergren NA; Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA., Bolling BG; Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA., Luo H; Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA., Singh A; Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA., Guzman H; Department of Pathology, University of Texas Medical Branch, Galveston, TX 77555, USA., Popov VL; Department of Pathology, University of Texas Medical Branch, Galveston, TX 77555, USA., Travassos da Rosa APA; Department of Pathology, University of Texas Medical Branch, Galveston, TX 77555, USA., Wang T; Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA., Kang L; Edward Via College of Osteopathic Medicine, Monroe, LA, 71203, USA; Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, Blacksburg, VA, 24060, USA., Allen IC; Center for Emerging, Zoonotic, and Arthropod-borne Pathogens, Virginia Polytechnic Institute and State University, Blacksburg, VA, 24061, USA; Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, Blacksburg, VA, 24060, USA., Carrington CVF; Department of Preclinical Sciences, Faculty of Medical Sciences, The University of the West Indies, St. Augustine, Trinidad and Tobago., Tesh RB; Department of Pathology, University of Texas Medical Branch, Galveston, TX 77555, USA., Weaver SC; Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA.
Jazyk: angličtina
Zdroj: Virology [Virology] 2021 Oct; Vol. 562, pp. 50-62. Date of Electronic Publication: 2021 Jul 08.
DOI: 10.1016/j.virol.2021.07.004
Abstrakt: We describe the isolation and characterization of a novel insect-specific flavivirus (ISFV), tentatively named Aripo virus (ARPV), that was isolated from Psorophora albipes mosquitoes collected in Trinidad. The ARPV genome was determined and phylogenetic analyses showed that it is a dual host associated ISFV, and clusters with the main mosquito-borne flaviviruses. ARPV antigen was significantly cross-reactive with Japanese encephalitis virus serogroup antisera, with significant cross-reactivity to Ilheus and West Nile virus (WNV). Results suggest that ARPV replication is limited to mosquitoes, as it did not replicate in the sandfly, culicoides or vertebrate cell lines tested. We also demonstrated that ARPV is endocytosed into vertebrate cells and is highly immunomodulatory, producing a robust innate immune response despite its inability to replicate in vertebrate systems. We show that prior infection or coinfection with ARPV limits WNV-induced disease in mouse models, likely the result of a robust ARPV-induced type I interferon response.
(Published by Elsevier Inc.)
Databáze: MEDLINE
Externí odkaz: