Photorhabdus antibacterial Rhs polymorphic toxin inhibits translation through ADP-ribosylation of 23S ribosomal RNA.

Autor:	Jurėnas D; Laboratoire d'Ingénierie des Systèmes Macromoléculaires (LISM), Institut de Microbiologie, Bioénergies et Biotechnologie (IM2B), Aix-Marseille Université - CNRS, UMR 7255, Marseille, France., Payelleville A; Laboratoire d'Ingénierie des Systèmes Macromoléculaires (LISM), Institut de Microbiologie, Bioénergies et Biotechnologie (IM2B), Aix-Marseille Université - CNRS, UMR 7255, Marseille, France.; DGIMI, Univ Montpellier, INRAE, Montpellier, France., Roghanian M; Department of Molecular Biology, Umeå University, 901 87 Umeå, Sweden.; Laboratory for Molecular Infection Medicine Sweden, Umeå University, 901 87 Umeå, Sweden., Turnbull KJ; Department of Molecular Biology, Umeå University, 901 87 Umeå, Sweden., Givaudan A; DGIMI, Univ Montpellier, INRAE, Montpellier, France., Brillard J; DGIMI, Univ Montpellier, INRAE, Montpellier, France., Hauryliuk V; Department of Molecular Biology, Umeå University, 901 87 Umeå, Sweden.; Laboratory for Molecular Infection Medicine Sweden, Umeå University, 901 87 Umeå, Sweden.; Department of Experimental Medical Science, Lund University, 221 00 Lund, Sweden.; University of Tartu, Institute of Technology, 50411 Tartu, Estonia., Cascales E; Laboratoire d'Ingénierie des Systèmes Macromoléculaires (LISM), Institut de Microbiologie, Bioénergies et Biotechnologie (IM2B), Aix-Marseille Université - CNRS, UMR 7255, Marseille, France.
Jazyk:	angličtina
Zdroj:	Nucleic acids research [Nucleic Acids Res] 2021 Aug 20; Vol. 49 (14), pp. 8384-8395.
DOI:	10.1093/nar/gkab608
Abstrakt:	Bacteria have evolved sophisticated mechanisms to deliver potent toxins into bacterial competitors or into eukaryotic cells in order to destroy rivals and gain access to a specific niche or to hijack essential metabolic or signaling pathways in the host. Delivered effectors carry various activities such as nucleases, phospholipases, peptidoglycan hydrolases, enzymes that deplete the pools of NADH or ATP, compromise the cell division machinery, or the host cell cytoskeleton. Effectors categorized in the family of polymorphic toxins have a modular structure, in which the toxin domain is fused to additional elements acting as cargo to adapt the effector to a specific secretion machinery. Here we show that Photorhabdus laumondii, an entomopathogen species, delivers a polymorphic antibacterial toxin via a type VI secretion system. This toxin inhibits protein synthesis in a NAD+-dependent manner. Using a biotinylated derivative of NAD, we demonstrate that translation is inhibited through ADP-ribosylation of the ribosomal 23S RNA. Mapping of the modification further showed that the adduct locates on helix 44 of the thiostrepton loop located in the GTPase-associated center and decreases the GTPase activity of the EF-G elongation factor. (© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.)
Databáze:	MEDLINE
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