Genome-wide analyses of human noroviruses provide insights on evolutionary dynamics and evidence of coexisting viral populations evolving under recombination constraints.

Autor: Tohma K; Division of Viral Products, CBER, FDA, Silver Spring, Maryland, United States of America., Lepore CJ; Division of Viral Products, CBER, FDA, Silver Spring, Maryland, United States of America., Martinez M; Division of Viral Products, CBER, FDA, Silver Spring, Maryland, United States of America.; IICS, National University of Asuncion, Asuncion, Paraguay., Degiuseppe JI; INEI-ANLIS, Ministry of Health, Buenos Aires, Argentina., Khamrin P; Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand., Saito M; Department of Virology, Tohoku University Graduate School of Medicine, Sendai, Japan., Mayta H; Department of Cellular and Molecular Sciences, Faculty of Sciences, Universidad Peruana Cayetano Heredia, Lima, Peru., Nwaba AUA; Division of Viral Products, CBER, FDA, Silver Spring, Maryland, United States of America., Ford-Siltz LA; Division of Viral Products, CBER, FDA, Silver Spring, Maryland, United States of America., Green KY; Laboratory of Infectious Diseases, NIAID, NIH, Bethesda, Maryland, United States of America., Galeano ME; IICS, National University of Asuncion, Asuncion, Paraguay., Zimic M; Department of Cellular and Molecular Sciences, Faculty of Sciences, Universidad Peruana Cayetano Heredia, Lima, Peru., Stupka JA; INEI-ANLIS, Ministry of Health, Buenos Aires, Argentina., Gilman RH; Department of International Health, Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland, United States of America., Maneekarn N; Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand., Ushijima H; Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Tokyo, Japan., Parra GI; Division of Viral Products, CBER, FDA, Silver Spring, Maryland, United States of America.
Jazyk: angličtina
Zdroj: PLoS pathogens [PLoS Pathog] 2021 Jul 13; Vol. 17 (7), pp. e1009744. Date of Electronic Publication: 2021 Jul 13 (Print Publication: 2021).
DOI: 10.1371/journal.ppat.1009744
Abstrakt: Norovirus is a major cause of acute gastroenteritis worldwide. Over 30 different genotypes, mostly from genogroup I (GI) and II (GII), have been shown to infect humans. Despite three decades of genome sequencing, our understanding of the role of genomic diversification across continents and time is incomplete. To close the spatiotemporal gap of genomic information of human noroviruses, we conducted a large-scale genome-wide analyses that included the nearly full-length sequencing of 281 archival viruses circulating since the 1970s in over 10 countries from four continents, with a major emphasis on norovirus genotypes that are currently underrepresented in public genome databases. We provided new genome information for 24 distinct genotypes, including the oldest genome information from 12 norovirus genotypes. Analyses of this new genomic information, together with those publicly available, showed that (i) noroviruses evolve at similar rates across genomic regions and genotypes; (ii) emerging viruses evolved from transiently-circulating intermediate viruses; (iii) diversifying selection on the VP1 protein was recorded in genotypes with multiple variants; (iv) non-structural proteins showed a similar branching on their phylogenetic trees; and (v) contrary to the current understanding, there are restrictions on the ability to recombine different genomic regions, which results in co-circulating populations of viruses evolving independently in human communities. This study provides a comprehensive genetic analysis of diverse norovirus genotypes and the role of non-structural proteins on viral diversification, shedding new light on the mechanisms of norovirus evolution and transmission.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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