CD40L modulates transcriptional signatures of neutrophils in the bone marrow associated with development and trafficking.

Autor: França TT; Department of Immunology, Institute of Biomedical Science, University of São Paulo, São Paulo, São Paulo, Brazil., Al-Sbiei A; Department of Medical Microbiology and Immunology, College of Medicine and Health Sciences, United Arab Emirates (UAE) University, Al Ain, Abu Dhabi, United Arab Emirates., Bashir G; Department of Medical Microbiology and Immunology, College of Medicine and Health Sciences, United Arab Emirates (UAE) University, Al Ain, Abu Dhabi, United Arab Emirates., Mohamed YA; Department of Medical Microbiology and Immunology, College of Medicine and Health Sciences, United Arab Emirates (UAE) University, Al Ain, Abu Dhabi, United Arab Emirates., Salgado RC; Department of Immunology, Institute of Biomedical Science, University of São Paulo, São Paulo, São Paulo, Brazil., Barreiros LA; Department of Immunology, Institute of Biomedical Science, University of São Paulo, São Paulo, São Paulo, Brazil., Maria da Silva Napoleão S; Department of Immunology, Institute of Biomedical Science, University of São Paulo, São Paulo, São Paulo, Brazil., Weber CW; Pediatric Allergy & Immunology Clinic, Caxias do Sul, Rio Grande do Sul, Brazil., Fernandes Severo Ferreira J; Albert Sabin Hospital, Fortaleza, Ceará, Brazil., Aranda CS; Division of Allergy, Immunology, and Rheumatology, Department of Pediatrics, Federal University of São Paulo, São Paulo, São Paulo, Brazil., Prando C; Faculdades Pequeno Príncipe, Pelé Pequeno Principe Research Intitute, Curitiba, Paraná, Brazil.; Hospital Pequeno Príncipe, Curitiba, Paraná, Brazil., de Barros Dorna MB; Division of Allergy and Immunology, Department of Pediatrics, Children's Institute, Hospital das Clínicas, São Paulo, São Paulo, Brazil., Jurisica I; Osteoarthritis Research Program, Division of Orthopedic Surgery, Schroeder Arthritis Institute, University Health Network, Krembil Research Institute, University Health Network, Departments of Medical Biophysics and Computer Science, University of Toronto, Toronto, Ontaro, Canada.; Institute of Neuroimmunology, Slovak Academy of Sciences, Bratislava, Slovakia., Fernandez-Cabezudo MJ; Department of Biochemistry and Molecular Biology, College of Medicine and Health Sciences, UAE University, Al Ain, Abu Dhabi, United Arab Emirates., Ochs HD; Department of Pediatrics, University of Washington School of Medicine, and Seattle Children's Research Institute, Seattle, Washington, USA., Condino-Neto A; Department of Immunology, Institute of Biomedical Science, University of São Paulo, São Paulo, São Paulo, Brazil., Al-Ramadi BK; Department of Medical Microbiology and Immunology, College of Medicine and Health Sciences, United Arab Emirates (UAE) University, Al Ain, Abu Dhabi, United Arab Emirates.; Zayed Center for Health Sciences, UAE University, Al Ain, Abu Dhabi, United Arab Emirates., Cabral-Marques O; Department of Immunology, Institute of Biomedical Science, University of São Paulo, São Paulo, São Paulo, Brazil.; Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, São Paulo, Brazil.; Network of Immunity in Infection, Malignancy, and Autoimmunity (NIIMA), Universal Scientific Education and Research Network (USERN), São Paulo, São Paulo, Brazil.
Jazyk: angličtina
Zdroj: JCI insight [JCI Insight] 2021 Aug 23; Vol. 6 (16). Date of Electronic Publication: 2021 Aug 23.
DOI: 10.1172/jci.insight.148652
Abstrakt: Neutrophils are produced in the BM in a process called granulopoiesis, in which progenitor cells sequentially develop into mature neutrophils. During the developmental process, which is finely regulated by distinct transcription factors, neutrophils acquire the ability to exit the BM, properly distribute throughout the body, and migrate to infection sites. Previous studies have demonstrated that CD40 ligand (CD40L) influences hematopoiesis and granulopoiesis. Here, we investigate the effect of CD40L on neutrophil development and trafficking by performing functional and transcriptome analyses. We found that CD40L signaling plays an essential role in the early stages of neutrophil generation and development in the BM. Moreover, CD40L modulates transcriptional signatures, indicating that this molecule enables neutrophils to traffic throughout the body and to migrate in response to inflammatory signals. Thus, our study provides insights into the complex relationships between CD40L signaling and granulopoiesis, and it suggests a potentially novel and nonredundant role of CD40L signaling in neutrophil development and function.
Databáze: MEDLINE