Single-cell profiling of CNS border compartment leukocytes reveals that B cells and their progenitors reside in non-diseased meninges.

Autor: Schafflick D; Department of Neurology with Institute of Translational Neurology, University Hospital Münster, Medical Faculty, Münster, Germany., Wolbert J; Department of Neurology with Institute of Translational Neurology, University Hospital Münster, Medical Faculty, Münster, Germany., Heming M; Department of Neurology with Institute of Translational Neurology, University Hospital Münster, Medical Faculty, Münster, Germany., Thomas C; Institute of Neuropathology, University Hospital Münster, Münster, Germany., Hartlehnert M; Department of Neurology with Institute of Translational Neurology, University Hospital Münster, Medical Faculty, Münster, Germany., Börsch AL; Department of Neurology with Institute of Translational Neurology, University Hospital Münster, Medical Faculty, Münster, Germany., Ricci A; Institute for Stroke and Dementia Research, University Hospital, LMU Munich, Munich, Germany., Martín-Salamanca S; Area of Cell and Developmental Biology, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Madrid, Spain., Li X; Department of Neurology with Institute of Translational Neurology, University Hospital Münster, Medical Faculty, Münster, Germany., Lu IN; Department of Neurology with Institute of Translational Neurology, University Hospital Münster, Medical Faculty, Münster, Germany., Pawlak M; Evergrande Center for Immunologic Diseases, Harvard Medical School and Brigham and Women's Hospital, Boston, MA, USA., Minnerup J; Department of Neurology with Institute of Translational Neurology, University Hospital Münster, Medical Faculty, Münster, Germany., Strecker JK; Department of Neurology with Institute of Translational Neurology, University Hospital Münster, Medical Faculty, Münster, Germany., Seidenbecher T; Institute for Physiology I, Westfälische Wilhelms-University Münster, Medical Faculty, Münster, Germany., Meuth SG; Department of Neurology with Institute of Translational Neurology, University Hospital Münster, Medical Faculty, Münster, Germany., Hidalgo A; Area of Cell and Developmental Biology, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Madrid, Spain., Liesz A; Institute for Stroke and Dementia Research, University Hospital, LMU Munich, Munich, Germany.; Munich Cluster for Systems Neurology (SyNergy), Munich, Germany., Wiendl H; Department of Neurology with Institute of Translational Neurology, University Hospital Münster, Medical Faculty, Münster, Germany., Meyer Zu Horste G; Department of Neurology with Institute of Translational Neurology, University Hospital Münster, Medical Faculty, Münster, Germany. gerd.meyerzuhoerste@ukmuenster.de.
Jazyk: angličtina
Zdroj: Nature neuroscience [Nat Neurosci] 2021 Sep; Vol. 24 (9), pp. 1225-1234. Date of Electronic Publication: 2021 Jul 12.
DOI: 10.1038/s41593-021-00880-y
Abstrakt: The CNS is ensheathed by the meninges and cerebrospinal fluid, and recent findings suggest that these CNS-associated border tissues have complex immunological functions. Unlike myeloid lineage cells, lymphocytes in border compartments have yet to be thoroughly characterized. Based on single-cell transcriptomics, we here identified a highly location-specific composition and expression profile of tissue-resident leukocytes in CNS parenchyma, pia-enriched subdural meninges, dura mater, choroid plexus and cerebrospinal fluid. The dura layer of the meninges contained a large population of B cells under homeostatic conditions in mice and rats. Murine dura B cells exhibited slow turnover and long-term tissue residency, and they matured in experimental neuroinflammation. The dura also contained B lineage progenitors at the pro-B cell stage typically not found outside of bone marrow, without direct influx from the periphery or the skull bone marrow. This identified the dura as an unexpected site of B cell residence and potentially of development in both homeostasis and neuroinflammation.
(© 2021. The Author(s), under exclusive licence to Springer Nature America, Inc.)
Databáze: MEDLINE
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