Formalin pre-fixation improves autopsy histology.
| Autor: | Vazzano J; The Ohio State University Wexner Medical Center, Department of Pathology, Columbus, OH, USA., Sinclair W; The Ohio State University Wexner Medical Center, Department of Pathology, Columbus, OH, USA., Zehr B; The Ohio State University Wexner Medical Center, Department of Pathology, Columbus, OH, USA., Allenby P; The Ohio State University Wexner Medical Center, Department of Pathology, Columbus, OH, USA. |
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| Jazyk: | angličtina |
| Zdroj: | Autopsy & case reports [Autops Case Rep] 2021 May 27; Vol. 11, pp. e2021291. Date of Electronic Publication: 2021 May 27 (Print Publication: 2021). |
| DOI: | 10.4322/acr.2021.291 |
| Abstrakt: | Microscopic findings in key tissues are often critical to determine the cause of death in medical autopsies. The overall quality of histologic sections depends on numerous pre-analytic factors, among which are tissue section size and thickness. We designed a prospective quality improvement study to determine whether a simple intervention of formalin pre-fixation of myocardium, liver, and kidney tissues could improve the ease of cutting and quality of autopsy histologic sections as assessed by histotechnicians and pathologists. Of 46 autopsies included in the study, 21 were randomly assigned to formalin pre-fixation, and 25 underwent routine sectioning without formalin pre-fixation. A significant improvement in overall quality score by histotechnicians was detected in the sections from pre-fixed autopsy tissues compared to the control group (p=0.0327). There was no significant difference in quality score between the two groups as assessed by pathologists. Our autopsy quality improvement study demonstrates that a simple, low-cost intervention of formalin pre-fixation of fresh autopsy tissues for 90 minutes could significantly improve the overall quality of sections submitted for histologic processing. Competing Interests: Conflict of interest: All authors have no conflicts of interest. (Copyright: © 2021 The Authors.) |
| Databáze: | MEDLINE |
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