A Simple in situ Assay to Assess Plant-Associative Bacterial Nitrogenase Activity.
| Autor: | Haskett TL; Department of Plant Sciences, University of Oxford, Oxford, United Kingdom., Knights HE; Department of Plant Sciences, University of Oxford, Oxford, United Kingdom., Jorrin B; Department of Plant Sciences, University of Oxford, Oxford, United Kingdom., Mendes MD; Department of Plant Sciences, University of Oxford, Oxford, United Kingdom., Poole PS; Department of Plant Sciences, University of Oxford, Oxford, United Kingdom. |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | Frontiers in microbiology [Front Microbiol] 2021 Jun 23; Vol. 12, pp. 690439. Date of Electronic Publication: 2021 Jun 23 (Print Publication: 2021). |
| DOI: | 10.3389/fmicb.2021.690439 |
| Abstrakt: | Assessment of plant-associative bacterial nitrogen (N) fixation is crucial for selection and development of elite diazotrophic inoculants that could be used to supply cereal crops with nitrogen in a sustainable manner. Although diazotrophic bacteria possess diverse oxygen tolerance mechanisms, most require a sub 21% oxygen environment to achieve optimal stability and function of the N-fixing catalyst nitrogenase. Consequently, assessment of N fixation is routinely carried out on "free-living" bacteria grown in the absence of a host plant and such experiments may not accurately divulge activity in the rhizosphere where the availability and forms of nutrients such as carbon and N, which are key regulators of N fixation, may vary widely. Here, we present a modified in situ acetylene reduction assay (ARA), utilizing the model cereal barley as a host to comparatively assess nitrogenase activity in diazotrophic bacteria. The assay is rapid, highly reproducible, applicable to a broad range of diazotrophs, and can be performed with simple equipment commonly found in most laboratories that investigate plant-microbe interactions. Thus, the assay could serve as a first point of order for high-throughput identification of elite plant-associative diazotrophs. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2021 Haskett, Knights, Jorrin, Mendes and Poole.) |
| Databáze: | MEDLINE |
| Externí odkaz: |
|