Hydrazone ligation assisted DNAzyme walking nanomachine coupled with CRISPR-Cas12a for lipopolysaccharide analysis.

Autor: Wang P; School of Life Sciences, Shanghai University, Shanghai, 200444, PR China., Liu Y; School of Life Sciences, Shanghai University, Shanghai, 200444, PR China., Yu Y; Department of Food Science and Engineering, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China., Zhang Y; School of Life Sciences, Shanghai University, Shanghai, 200444, PR China., Peng J; Department of Joint Surgery and Orthopedic Medicine, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai, 200003, PR China., Niu L; School of Life Sciences, Shanghai University, Shanghai, 200444, PR China., Zhang J; School of Life Sciences, Shanghai University, Shanghai, 200444, PR China. Electronic address: juanzhang@shu.edu.cn.
Jazyk: angličtina
Zdroj: Analytica chimica acta [Anal Chim Acta] 2021 Aug 22; Vol. 1174, pp. 338747. Date of Electronic Publication: 2021 Jun 08.
DOI: 10.1016/j.aca.2021.338747
Abstrakt: In this work, hydrazone ligation assisted DNAzyme walking nanomachine is explored to couple with CRISPR-Cas12a trans-cleavage. Hydrazone ligation with high efficiency can mediate signal input which can be induced by target binding, thereby regulating the performance of DNAzyme walking nanomachine. The product strand from DNAzyme walking nanomachine can further activate the trans-cleavage of Cas12a. So, cascade signal amplification can be achieved to enhance the sensitivity for target detection. Subsequently, hydrazone ligation assisted DNAzyme walking nanomachine coupled with CRISPR-Cas12a has been further developed as a biosensor to analyze lipopolysaccharides. The developed biosensor exhibits a linear range from 0.05 ng/mL to 10 6  ng/mL and a lowest limit of detection of 7.31 fg/mL. This research provides a new mode for the signal output of DNAzyme walking nanomachine, so as to sensitively analyze different biomolecules.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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