Effects of antiviral drug therapy on dynamic thiol/disulphide homeostasis and nitric oxide levels in COVID-19 patients.

Autor: Mete AÖ; Department of Infectious Diseases and Clinical Microbiology, Faculty of Medicine, Gaziantep University, Gaziantep, Turkey. Electronic address: ozlemmete@gantep.edu.tr., Koçak K; Department of Infectious Diseases and Clinical Microbiology, Faculty of Medicine, Gaziantep University, Gaziantep, Turkey., Saracaloglu A; Department of Medical Pharmacology, Faculty of Medicine, Gaziantep University, Gaziantep, Turkey., Demiryürek S; Department of Physiology, Faculty of Medicine, Gaziantep University, Gaziantep, Turkey., Altınbaş Ö; Department of Operating Room Services, Vocational School of Health Services, Gaziantep University, Gaziantep, Turkey., Demiryürek AT; Department of Medical Pharmacology, Faculty of Medicine, Gaziantep University, Gaziantep, Turkey; Vocational School of Health Services, Gaziantep University, Gaziantep, Turkey.
Jazyk: angličtina
Zdroj: European journal of pharmacology [Eur J Pharmacol] 2021 Sep 15; Vol. 907, pp. 174306. Date of Electronic Publication: 2021 Jul 07.
DOI: 10.1016/j.ejphar.2021.174306
Abstrakt: The novel coronavirus disease 2019 (COVID-19) has led to a serious global pandemic. Although an oxidative stress imbalance occurs in COVID-19 patients, the contributions of thiol/disulphide homeostasis and nitric oxide (NO) generation to the pathogenesis of COVID-19 have been poorly identified. Therefore, the aim of this study was to evaluate the effects of antiviral drug therapy on the serum dynamics of thiol/disulphide homeostasis and NO levels in COVID-19 patients. A total of 50 adult patients with COVID-19 and 43 sex-matched healthy control subjects were enrolled in this prospective study. Venous blood samples were collected immediately on admission to the hospital within 24 h after the diagnosis (pre-treatment) and at the 15th day of drug therapy (post-treatment). Serum native thiol and total thiol levels were measured, and the amounts of dynamic disulphide bonds and related ratios were calculated. The average pre-treatment total and native thiol levels were significantly lower than the post-treatment values (P < 0.001 for all). We observed no significant changes in disulphide levels or disulphide/total thiol, disulphide/native thiol, or native thiol/total thiol ratios between pre- and post-treatments. There was also a significant increase in serum NO levels in the pre-treatment values when compared to control (P < 0.001) and post-treatment measurements (P < 0.01). Our results strongly suggest that thiol/disulphide homeostasis and nitrosative stress can contribute to the pathogenesis of COVID-19. This study was the first to show that antiviral drug therapy can prevent the depletion in serum thiol levels and decrease serum NO levels in COVID-19 patients.
(Copyright © 2021. Published by Elsevier B.V.)
Databáze: MEDLINE
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