Regional specific differentiation of integumentary organs: SATB2 is involved in α- and β-keratin gene cluster switching in the chicken.

Autor: Lin GW; Department of Pathology, Keck School of Medicine, University of Southern California, Los Angeles, California, USA.; Department of Biochemistry and Molecular Cell Biology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan., Liang YC; Department of Pathology, Keck School of Medicine, University of Southern California, Los Angeles, California, USA.; Integrative Stem Cell Center, China Medical University and Hospital, China Medical University, Taichung, Taiwan., Wu P; Department of Pathology, Keck School of Medicine, University of Southern California, Los Angeles, California, USA., Chen CK; Department of Pathology, Keck School of Medicine, University of Southern California, Los Angeles, California, USA.; The IEGG and Animal Biotechnology Center, National Chung Hsing University, Taichung, Taiwan., Lai YC; Department of Pathology, Keck School of Medicine, University of Southern California, Los Angeles, California, USA.; Integrative Stem Cell Center, China Medical University and Hospital, China Medical University, Taichung, Taiwan.; Institute of New Drug Development, China Medical University, Taichung, Taiwan., Jiang TX; Department of Pathology, Keck School of Medicine, University of Southern California, Los Angeles, California, USA., Haung YH; Department of Biochemistry and Molecular Cell Biology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.; TMU Research Center of Cell Therapy and Regeneration Medicine, Taipei Medical University, Taipei, Taiwan., Chuong CM; Department of Pathology, Keck School of Medicine, University of Southern California, Los Angeles, California, USA.
Jazyk: angličtina
Zdroj: Developmental dynamics : an official publication of the American Association of Anatomists [Dev Dyn] 2022 Sep; Vol. 251 (9), pp. 1490-1508. Date of Electronic Publication: 2021 Jul 17.
DOI: 10.1002/dvdy.396
Abstrakt: Background: Animals develop skin regional specificities to best adapt to their environments. Birds are excellent models in which to study the epigenetic mechanisms that facilitate these adaptions. Patients suffering from SATB2 mutations exhibit multiple defects including ectodermal dysplasia-like changes. The preferential expression of SATB2, a chromatin regulator, in feather-forming compared to scale-forming regions, suggests it functions in regional specification of chicken skin appendages by acting on either differentiation or morphogenesis.
Results: Retrovirus mediated SATB2 misexpression in developing feathers, beaks, and claws causes epidermal differentiation abnormalities (e.g. knobs, plaques) with few organ morphology alterations. Chicken β-keratins are encoded in 5 sub-clusters (Claw, Feather, Feather-like, Scale, and Keratinocyte) on Chromosome 25 and a large Feather keratin cluster on Chromosome 27. Type I and II α-keratin clusters are located on Chromosomes 27 and 33, respectively. Transcriptome analyses showed these keratins (1) are often tuned up or down collectively as a sub-cluster, and (2) these changes occur in a temporo-spatial specific manner.
Conclusions: These results suggest an organizing role of SATB2 in cluster-level gene co-regulation during skin regional specification.
(© 2021 American Association for Anatomy.)
Databáze: MEDLINE
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