Gingko biloba abrogate lead-induced neurodegeneration in mice hippocampus: involvement of NF-κB expression, myeloperoxidase activity and pro-inflammatory mediators.

Autor: Adebayo OG; Neurophysiology Unit, Department of Physiology, PAMO University of Medical Sciences, Port-Harcourt, River State, Nigeria. segsyn07@gmail.com., Ben-Azu B; Department of Pharmacology and Therapeutics, Faculty of Basic Medical Sciences, College of Health Sciences, Delta State University, Abraka, Delta State, Nigeria. pharmben4ever@yahoo.com., Ajayi AM; Department of Pharmacology and Therapeutics, Faculty of Basic Medical Sciences, University of Ibadan, Ibadan, Oyo State, Nigeria., Wopara I; Department of Biochemistry, Faculty of Basic Medical Sciences, University of Port Harcourt, Port Harcourt, River State, Nigeria., Aduema W; Department of Physiology, Bayelsa Medical University, Yenagoa, Bayelsa State, Nigeria., Kolawole TA; Neurophysiology Unit, Department of Physiology, PAMO University of Medical Sciences, Port-Harcourt, River State, Nigeria., Umoren EB; Neurophysiology Unit, Department of Physiology, PAMO University of Medical Sciences, Port-Harcourt, River State, Nigeria., Onyeleonu I; Department of Anatomy, PAMO University of Medical Sciences, Port-Harcourt, River State, Nigeria., Ebo OT; Department of Community Medicine, Faculty of Clinical Sciences, College of Medicine, University of Ibadan, Ibadan, Nigeria., Ajibo DN; Department of Pharmacy, Faculty of Clinical Sciences, University of Port Harcourt, Port Harcourt, River State, Nigeria., Akpotu AE; Department of Pharmacology and Therapeutics, College of Medicine, University of Nigeria, Enugu, Enugu State, Nigeria.
Jazyk: angličtina
Zdroj: Biological trace element research [Biol Trace Elem Res] 2022 Apr; Vol. 200 (4), pp. 1736-1749. Date of Electronic Publication: 2021 Jul 09.
DOI: 10.1007/s12011-021-02790-3
Abstrakt: Neuroimmune alterations have important implication in the neuropsychiatric symptoms and biochemical changes associated with lead-induced neurotoxicity. It has been suggested that inhibition of neuroinflammatory-mediated lead-induced neurotoxicity by phytochemicals enriched with antioxidant activities would attenuate the deleterious effects caused by lead. Hence, this study investigated the neuroinflammatory mechanism behind the effect of Ginkgo biloba supplement (GB-S) in lead-induced neurotoxicity in mice brains. Mice were intraperitoneally pretreated with lead acetate (100 mg/kg) for 30 min prior the administration of GB-S (10 and 20 mg/kg, i.p.) and ethylenediaminetetraacetic acid (EDTA) (50 mg/kg, i.p.) for 14 consecutive days. Symptoms of neurobehavioral impairment were evaluated using open field test (OFT), elevated plus maze (EPM), and tail suspension test (TST) respectively. Thereafter, mice brain hippocampi were sectioned for myeloperoxidase activity (MPO), pro-inflammatory cytokine (TNF-α and IL-6) estimation and inflammatory protein (NF-κB) expression. Furthermore, histomorphormetric studies (Golgi impregnation and Cresyl violet stainings) were carried out. GB-S (10 and 20 mg/kg) significantly restores neurobehavioral impairments based on improved locomotion, reduced anxiety- and depressive-like behavior. Moreover, GB-S reduced the MPO activity, inhibits TNF-α, IL-6 release, and downregulates NF-κB immunopositive cell expression in mice hippocampus. Histomorphometrically, GB-S also prevents the loss of pyramidal neuron in the hippocampus. The endpoint of this findings suggest that GB-S decreases neuropsychiatric symptoms induced by lead acetate through mechanisms related to inhibition of release of pro-inflammatory mediators and suppression of hippocampal pyramidal neuron degeneration in mice.
(© 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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