Dual dean entrainment with volume ratio modulation for efficient droplet co-encapsulation: extreme single-cell indexing.

Autor:	Harrington J; Cancer Sciences, Faculty of Medicine, University of Southampton, UK. J.J.West@soton.ac.uk., Esteban LB; Faculty of Engineering and Physical Sciences, University of Southampton, UK., Butement J; Cancer Sciences, Faculty of Medicine, University of Southampton, UK. J.J.West@soton.ac.uk., Vallejo AF; Clinical and Experimental Sciences, Sir Henry Wellcome Laboratories, Faculty of Medicine, University of Southampton, UK., Lane SIR; Faculty of Engineering and Physical Sciences, University of Southampton, UK.; Institute for Life Sciences, University of Southampton, UK., Sheth B; School for Biological Sciences, Faculty of Environmental and Life Sciences, University of Southampton, UK., Jongen MSA; Cancer Sciences, Faculty of Medicine, University of Southampton, UK. J.J.West@soton.ac.uk., Parker R; Cancer Sciences, Faculty of Medicine, University of Southampton, UK. J.J.West@soton.ac.uk., Stumpf PS; Centre for Human Development, Stem Cells and Regeneration, Faculty of Medicine, University of Southampton, UK., Smith RCG; Cancer Sciences, Faculty of Medicine, University of Southampton, UK. J.J.West@soton.ac.uk., MacArthur BD; Institute for Life Sciences, University of Southampton, UK.; Mathematical Sciences, University of Southampton, UK., Rose-Zerilli MJJ; Cancer Sciences, Faculty of Medicine, University of Southampton, UK. J.J.West@soton.ac.uk.; Institute for Life Sciences, University of Southampton, UK., Polak ME; Clinical and Experimental Sciences, Sir Henry Wellcome Laboratories, Faculty of Medicine, University of Southampton, UK.; Institute for Life Sciences, University of Southampton, UK., Underwood T; Cancer Sciences, Faculty of Medicine, University of Southampton, UK. J.J.West@soton.ac.uk.; Institute for Life Sciences, University of Southampton, UK., West J; Cancer Sciences, Faculty of Medicine, University of Southampton, UK. J.J.West@soton.ac.uk.; Institute for Life Sciences, University of Southampton, UK.
Jazyk:	angličtina
Zdroj:	Lab on a chip [Lab Chip] 2021 Sep 07; Vol. 21 (17), pp. 3378-3386. Date of Electronic Publication: 2021 Jul 09.
DOI:	10.1039/d1lc00292a
Abstrakt:	The future of single cell diversity screens involves ever-larger sample sizes, dictating the need for higher throughput methods with low analytical noise to accurately describe the nature of the cellular system. Current approaches are limited by the Poisson statistic, requiring dilute cell suspensions and associated losses in throughput. In this contribution, we apply Dean entrainment to both cell and bead inputs, defining different volume packets to effect efficient co-encapsulation. Volume ratio scaling was explored to identify optimal conditions. This enabled the co-encapsulation of single cells with reporter beads at rates of ∼1 million cells per hour, while increasing assay signal-to-noise with cell multiplet rates of ∼2.5% and capturing ∼70% of cells. The method, called Pirouette coupling, extends our capacity to investigate biological systems.
Databáze:	MEDLINE
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