In Vitro Effects of Propofol on Cytotoxic, Apoptotic and PI3K-Akt Signaling Pathway Genes on Brain Cancer Cells.

Autor:	Edgunlu TG; Department of Medical Biology, Faculty of Medicine, Muğla Sıtkı Koçman University, Muğla, Turkey., Avci CB; Department of Medical Biology, Faculty of Medicine, Ege University, Bornava, Turkey., Ozates NP; Department of Medical Biology, Faculty of Medicine, Ege University, Bornava, Turkey., Bagca BG; Department of Medical Biology, Faculty of Medicine, Ege University, Bornava, Turkey., Celik SK; Department of Medical Genetic, Faculty of Medicine, Bülent Ecevit University, Zonguldak, Turkey., Boluk A; Faculty of Medicine, Muğla Sıtkı Koçman University, Muğla, Turkey., Ugur B; Department of Anesthesiology and Reanimation, Faculty of Medicine, Muğla Sıtkı Koçman University, Muğla, Turkey.
Jazyk:	angličtina
Zdroj:	Anti-cancer agents in medicinal chemistry [Anticancer Agents Med Chem] 2022; Vol. 22 (2), pp. 356-361.
DOI:	10.2174/1871520621666210708094328
Abstrakt:	Aim: The study aimed to determine the cytotoxic and apoptotic effect of propofol on glioma cells. Background: Propofol [2,6-diisopropylphenol] is a commonly used intravenous anesthetic. Propofol is known to have a mechanism of action on the PI3K-AKT pathway. Objective: This study aimed to evaluate the effect of propofol on the proliferation and apoptosis of human glioma cells, as well as to investigate changes in expression levels of the PI3K-AKT signaling pathway genes. Materials and Methods: The cytotoxic effect of propofol on the U-87 MG cell line was determined by WST-1 method. Annexin V-FITC and Mitoprobe JC-1 assay were used to measure apoptosis by flow cytometry. The expression levels of genes in the PI3K-AKT signaling pathway were investigated by qRT-PCR. Results: We have shown that propofol induced apoptosis in U-87 MG cells by 17.1 fold compared to the untreated control. Furthermore, significant differences were found in the expression levels of the PI3K-AKT signaling pathway genes. Conclusion: As a result of our study, it was found that propofol caused differences in expression levels of PI3K-AKT signaling pathway genes and it was suggested that these differences may be related to apoptosis induction. (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu