| Autor: |
Aguirre Carrión PJ; Department of Biology, Boston University, Boston, MA, USA., Williams LM; Department of Biology, Boston University, Boston, MA, USA., Gilmore TD; Department of Biology, Boston University, Boston, MA, USA. gilmore@bu.edu. |
| Jazyk: |
angličtina |
| Zdroj: |
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2366, pp. 67-91. |
| DOI: |
10.1007/978-1-0716-1669-7_5 |
| Abstrakt: |
Extensive genomic and transcriptomic sequencing over the past decade has revealed NF-κB signaling pathway homologs in organisms basal to insects, for example, in members of the phyla Cnidaria (e.g., sea anemones, corals, hydra, jellyfish) and Porifera (sponges), and in several single-celled protists (e.g., Capsaspora owczarzaki, some choanoflagellates). Therefore, methods are required to study the function of NF-κB and its pathway members in early branching organisms, many of which do not have histories as model organisms. Here, we describe a combination of cellular, molecular, and biochemical techniques that have been used for studying NF-κB, and related pathway proteins, in some of these basal organisms. These methods are useful for studying the evolution of NF-κB signaling, and may be adaptable to the study of NF-κB in other non-model organisms. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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