Translatomic analysis of regenerating and degenerating spinal motor neurons in injury and ALS.

Autor:	Shadrach JL; Department of Biologic and Materials Sciences, University of Michigan, Ann Arbor, MI, USA.; Cellular and Molecular Biology Graduate Program, University of Michigan, Ann Arbor, MI, USA., Stansberry WM; Department of Anatomy, Cell Biology & Physiology, Stark Neurosciences Research Institute, Indiana University School of Medicine, Indianapolis, IN, USA., Milen AM; Department of Biologic and Materials Sciences, University of Michigan, Ann Arbor, MI, USA., Ives RE; Department of Biologic and Materials Sciences, University of Michigan, Ann Arbor, MI, USA., Fogarty EA; Department of Human Genetics, University of Michigan, Ann Arbor, MI, USA., Antonellis A; Neuroscience Graduate Program, University of Michigan, Ann Arbor, MI, USA.; Department of Human Genetics, University of Michigan, Ann Arbor, MI, USA.; Department of Neurology, University of Michigan, Ann Arbor, MI, USA., Pierchala BA; Department of Biologic and Materials Sciences, University of Michigan, Ann Arbor, MI, USA.; Cellular and Molecular Biology Graduate Program, University of Michigan, Ann Arbor, MI, USA.; Neuroscience Graduate Program, University of Michigan, Ann Arbor, MI, USA.; Department of Anatomy, Cell Biology & Physiology, Stark Neurosciences Research Institute, Indiana University School of Medicine, Indianapolis, IN, USA.
Jazyk:	angličtina
Zdroj:	IScience [iScience] 2021 Jun 08; Vol. 24 (7), pp. 102700. Date of Electronic Publication: 2021 Jun 08 (Print Publication: 2021).
DOI:	10.1016/j.isci.2021.102700
Abstrakt:	The neuromuscular junction is a synapse critical for muscle strength and coordinated motor function. Unlike CNS injuries, motor neurons mount robust regenerative responses after peripheral nerve injuries. Conversely, motor neurons selectively degenerate in diseases such as amyotrophic lateral sclerosis (ALS). To assess how these insults affect motor neurons in vivo , we performed ribosomal profiling of mouse motor neurons. Motor neuron-specific transcripts were isolated from spinal cords following sciatic nerve crush, a model of acute injury and regeneration, and in the SOD1 G93A ALS model. Of the 267 transcripts upregulated after nerve crush, 38% were also upregulated in SOD1 G93A motor neurons. However, most upregulated genes in injured and ALS motor neurons were context specific. Some of the most significantly upregulated transcripts in both paradigms were chemokines such as Ccl2 and Ccl7 , suggesting an important role for neuroimmune modulation. Collectively these data will aid in defining pro-regenerative and pro-degenerative mechanisms in motor neurons. Competing Interests: The authors declare no competing interests. (© 2021 The Authors.)
Databáze:	MEDLINE
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