Novel greenly synthesized titanium dioxide nanoparticles compared to liposomes in drug delivery: in vivo investigation on Ehrlich solid tumor model.

Autor: Abdel Fadeel DA; Pharmaceutical Technology Unit, Department of Medical Applications of Laser, National Institute of Laser Enhanced Sciences, Cairo University, Cairo, Egypt., Hanafy MS; Biophysics Branch, Physics Department, Faculty of Science, Zagazig University, 44519 Zagazig, Egypt., Kelany NA; Biophysics Branch, Physics Department, Faculty of Science, Zagazig University, 44519 Zagazig, Egypt., Elywa MA; Biophysics Branch, Physics Department, Faculty of Science, Zagazig University, 44519 Zagazig, Egypt.
Jazyk: angličtina
Zdroj: Heliyon [Heliyon] 2021 Jun 21; Vol. 7 (6), pp. e07370. Date of Electronic Publication: 2021 Jun 21 (Print Publication: 2021).
DOI: 10.1016/j.heliyon.2021.e07370
Abstrakt: Aims: In a previous work, a pure crystalline titanium dioxide nanoparticles (TiO 2 NPs) were synthesized by green synthesis technique using Aloe vera leaves extract as reducing agent. In this work, we are aiming to investigate the potential of the novel greenly synthesized TiO 2 NPs as a nano-drug delivery system for the anticancer drug, doxorubicin (Dox).
Main Methods: The cytotoxicity of the synthesized TiO 2 NPs was tested on two cell lines; normal human skin fibroblasts (HSF) and breast adenocarcinoma cells (MCF-7). Then, Dox was loaded to both TiO 2 NPs (Dox- TiO 2 NPs) and liposomes (Dox-Lip). The loaded nanoparticles were characterized by TEM, FTIR, encapsulation efficiency, particle size and zeta potential measurement. Moreover, in vitro drug release was studied. Ehrlich tumor-bearing mice were used to study the anticancer activity of Dox- TiO 2 NPs, Dox-Lip, and aqueous Dox solution. Tumor volume, survival rate, and histopathological alterations were compared in all groups.
Key Findings: Dox was successfully loaded to both liposomes and TiO 2 NPs with an encapsulation efficiency of 77% and 65%, respectively. The particle size of Dox-TiO 2 NPs, and Dox-Lip was 14.53 nm, and 103 nm, respectively. The cumulative Dox released from TiO 2 NPs and liposomes after 4 h was 18 and 46%, respectively.Dox-Lip and Dox-TiO 2 NPs resulted in the highest degree of tumor growth inhibition with 100% and 83% of treated animals remained alive, respectively.
Significance: The greenly synthesized TiO 2 NPs were proved to be as effective as liposomes in enhancing the anticancer activity of Dox.
Competing Interests: The authors declare no conflict of interest.
(© 2021 The Author(s).)
Databáze: MEDLINE
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