Acceleration of age-induced proteolysis in the guinea pig lens nucleus by in vivo exposure to hyperbaric oxygen: A mass spectrometry analysis.

Autor: Giblin FJ; Eye Research Institute, Oakland University, Rochester, MI, 48309, USA., Anderson DMG; Department of Biochemistry and Mass Spectrometry Research Center, Vanderbilt University, Nashville, TN, 37242, USA., Han J; Genome BC Proteomics Centre, University of Victoria, Victoria, British Columbia, Canada., Rose KL; Department of Biochemistry and Mass Spectrometry Research Center, Vanderbilt University, Nashville, TN, 37242, USA., Wang Z; Department of Biochemistry and Mass Spectrometry Research Center, Vanderbilt University, Nashville, TN, 37242, USA., Schey KL; Department of Biochemistry and Mass Spectrometry Research Center, Vanderbilt University, Nashville, TN, 37242, USA. Electronic address: kevin.schey@vanderbilt.edu.
Jazyk: angličtina
Zdroj: Experimental eye research [Exp Eye Res] 2021 Sep; Vol. 210, pp. 108697. Date of Electronic Publication: 2021 Jul 04.
DOI: 10.1016/j.exer.2021.108697
Abstrakt: Hyperbaric oxygen (HBO) treatment of animals or ocular lenses in culture recapitulates many molecular changes observed in human age-related nuclear cataract. The guinea pig HBO model has been one of the best examples of such treatment leading to dose-dependent development of lens nuclear opacities. In this study, complimentary mass spectrometry methods were employed to examine protein truncation after HBO treatment of aged guinea pigs. Quantitative liquid chromatography-mass spectrometry (LC-MS) analysis of the membrane fraction of guinea pig lenses showed statistically significant increases in aquaporin-0 (AQP0) C-terminal truncation, consistent with previous reports of accelerated loss of membrane and cytoskeletal proteins. In addition, imaging mass spectrometry (IMS) analysis spatially mapped the acceleration of age-related αA-crystallin truncation in the lens nucleus. The truncation sites in αA-crystallin closely match those observed in human lenses with age. Taken together, our results suggest that HBO accelerates the normal lens aging process and leads to nuclear cataract.
(Copyright © 2021 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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