Type VI secretion system killing by commensal Neisseria is influenced by expression of type four pili.

Autor:	Custodio R; Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom., Ford RM; Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom., Ellison CJ; Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom., Liu G; Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom., Mickute G; Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom., Tang CM; Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom., Exley RM; Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2021 Jul 07; Vol. 10. Date of Electronic Publication: 2021 Jul 07.
DOI:	10.7554/eLife.63755
Abstrakt:	Type VI Secretion Systems (T6SSs) are widespread in bacteria and can dictate the development and organisation of polymicrobial ecosystems by mediating contact dependent killing. In Neisseria species, including Neisseria cinerea a commensal of the human respiratory tract, interbacterial contacts are mediated by Type four pili (Tfp) which promote formation of aggregates and govern the spatial dynamics of growing Neisseria microcolonies. Here, we show that N. cinerea expresses a plasmid-encoded T6SS that is active and can limit growth of related pathogens. We explored the impact of Tfp on N. cinerea T6SS-dependent killing within a colony and show that pilus expression by a prey strain enhances susceptibility to T6SS compared to a non-piliated prey, by preventing segregation from a T6SS-wielding attacker. Our findings have important implications for understanding how spatial constraints during contact-dependent antagonism can shape the evolution of microbial communities. Competing Interests: RC, RF, CE, GL, GM, CT No competing interests declared, RE Previous Member of the Scientific Advisory Panel of Meningitis Research Foundation (until 2021) (© 2021, Custodio et al.)
Databáze:	MEDLINE
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