Resistance to Durvalumab and Durvalumab plus Tremelimumab Is Associated with Functional STK11 Mutations in Patients with Non-Small Cell Lung Cancer and Is Reversed by STAT3 Knockdown.

Autor:	Pore N; AstraZeneca, Gaithersburg, Maryland., Wu S; AstraZeneca, Gaithersburg, Maryland., Standifer N; AstraZeneca, Gaithersburg, Maryland., Jure-Kunkel M; AstraZeneca, Gaithersburg, Maryland., de Los Reyes M; AstraZeneca, Gaithersburg, Maryland., Shrestha Y; AstraZeneca, Gaithersburg, Maryland., Halpin R; AstraZeneca, Gaithersburg, Maryland., Rothstein R; AstraZeneca, Gaithersburg, Maryland., Mulgrew K; AstraZeneca, Gaithersburg, Maryland., Blackmore S; AstraZeneca, Gaithersburg, Maryland., Martin P; AstraZeneca, Gaithersburg, Maryland., Meekin J 3rd; AstraZeneca, Gaithersburg, Maryland., Griffin M; AstraZeneca, Waltham, Massachusetts., Bisha I; AstraZeneca, Munich, Germany., Proia TA; AstraZeneca, Waltham, Massachusetts., Miragaia RJ; AstraZeneca, Cambridge, United Kingdom., Herbst R; AstraZeneca, Gaithersburg, Maryland., Gupta A; AstraZeneca, Gaithersburg, Maryland., Abdullah SE; AstraZeneca, Gaithersburg, Maryland., Raja R; AstraZeneca, Gaithersburg, Maryland., Frigault MM; AstraZeneca, Gaithersburg, Maryland., Barrett JC; AstraZeneca, Gaithersburg, Maryland., Dennis PA; AstraZeneca, Gaithersburg, Maryland., Ascierto ML; AstraZeneca, Gaithersburg, Maryland. ml.ascierto@gmailcom michael.oberst@astrazeneca.com., Oberst MD; AstraZeneca, Gaithersburg, Maryland. ml.ascierto@gmailcom michael.oberst@astrazeneca.com.
Jazyk:	angličtina
Zdroj:	Cancer discovery [Cancer Discov] 2021 Nov; Vol. 11 (11), pp. 2828-2845. Date of Electronic Publication: 2021 Jul 06.
DOI:	10.1158/2159-8290.CD-20-1543
Abstrakt:	Mutations in the STK11 (LKB1) gene regulate resistance to PD-1/PD-L1 blockade. This study evaluated this association in patients with nonsquamous non-small cell lung cancer (NSCLC) enrolled in three phase I/II trials. STK11 mutations were associated with resistance to the anti-PD-L1 antibody durvalumab (alone/with the anti-CTLA4 antibody tremelimumab) independently of KRAS mutational status, highlighting STK11 as a potential driver of resistance to checkpoint blockade. Retrospective assessments of tumor tissue, whole blood, and serum revealed a unique immune phenotype in patients with STK11 mutations, with increased expression of markers associated with neutrophils (i.e., CXCL2 , IL6 ), Th17 contexture (i.e., IL17A ), and immune checkpoints. Associated changes were observed in the periphery. Reduction of STAT3 in the tumor microenvironment using an antisense oligonucleotide reversed immunotherapy resistance in preclinical STK11 knockout models. These results suggest that STK11 mutations may hinder response to checkpoint blockade through mechanisms including suppressive myeloid cell biology, which could be reversed by STAT3-targeted therapy. SIGNIFICANCE: Patients with nonsquamous STK11 -mutant ( STK11 mut) NSCLC are less likely than STK11 wild-type ( STK11 wt) patients to respond to anti-PD-L1 ± anti-CTLA4 immunotherapies, and their tumors show increased expression of genes and cytokines that activate STAT3 signaling. Preclinically, STAT3 modulation reverses this resistance, suggesting STAT3-targeted agents as potential combination partners for immunotherapies in STK11 mut NSCLC. This article is highlighted in the In This Issue feature, p. 2659 . (©2021 The Authors; Published by the American Association for Cancer Research.)
Databáze:	MEDLINE
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