UV-induced Zn:Cd/S quantum dots in-situ formed in the presence of thiols for sensitive and selective fluorescence detection of thiols.

Autor:	Assi N; Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, 613 00, Brno, Czech Republic.; Central European Institute of Technology, Brno University of Technology, Purkynova 123, 612 00, Brno, Czech Republic., Nejdl L; Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, 613 00, Brno, Czech Republic.; Central European Institute of Technology, Brno University of Technology, Purkynova 123, 612 00, Brno, Czech Republic., Zemankova K; Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, 613 00, Brno, Czech Republic.; Central European Institute of Technology, Brno University of Technology, Purkynova 123, 612 00, Brno, Czech Republic., Pavelicova K; Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, 613 00, Brno, Czech Republic.; Central European Institute of Technology, Brno University of Technology, Purkynova 123, 612 00, Brno, Czech Republic., Bezdekova J; Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, 613 00, Brno, Czech Republic.; Central European Institute of Technology, Brno University of Technology, Purkynova 123, 612 00, Brno, Czech Republic., Macka M; Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, 613 00, Brno, Czech Republic.; Central European Institute of Technology, Brno University of Technology, Purkynova 123, 612 00, Brno, Czech Republic., Adam V; Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, 613 00, Brno, Czech Republic.; Central European Institute of Technology, Brno University of Technology, Purkynova 123, 612 00, Brno, Czech Republic., Vaculovicova M; Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, 613 00, Brno, Czech Republic. marketa.ryvolova@seznam.cz.; Central European Institute of Technology, Brno University of Technology, Purkynova 123, 612 00, Brno, Czech Republic. marketa.ryvolova@seznam.cz.
Jazyk:	angličtina
Zdroj:	Scientific reports [Sci Rep] 2021 Jul 05; Vol. 11 (1), pp. 13806. Date of Electronic Publication: 2021 Jul 05.
DOI:	10.1038/s41598-021-93137-4
Abstrakt:	In this work, we explored a new approach to a simple and sensitive fluorescence detection of thiols. The approach takes advantage of an in-situ formation of UV light-induced fluorescent nanoparticles (ZnCd/S quantum dots), while utilizing the thiol group of the analyte as a capping agent. The selectivity is ensured by the selective isolation of the thiol analyte by a polydopamine molecularly imprinted polymeric (MIP) layer. Based on this approach, a method for determination of thiols was designed. Key experimental parameters were optimized, including those of molecular imprinting and of effective model thiol molecule (L-cysteine) isolation. The relationship between the fluorescence intensity of ZnCd/S quantum dots and the concentration of L-cysteine in the range of 12-150 µg/mL was linear with a detection limit of 3.6 µg/mL. The molecularly imprinted polymer showed high absorption mass capacity (1.73 mg/g) and an excellent selectivity factor for L-cysteine compared to N-acetyl-L-cysteine and L-homocysteine of 63.56 and 87.48, respectively. The proposed method was applied for L-cysteine determination in human urine with satisfactory results. Due to a high variability of molecular imprinting technology and versatility of in-situ probe formation, methods based on this approach can be easily adopted for analysis of any thiol of interest.
Databáze:	MEDLINE
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