Genotyping and lipid profiling of 601 cultivated sunflower lines reveals novel genetic determinants of oil fatty acid content.

Autor: Chernova AI; Skolkovo Institute of Science and Technology (Skoltech), Bolshoy Boulevard 30, bld. 1, Moscow, 121205, Russia. alin.chernova@gmail.com.; LLC 'OIL GENE', Skolkovo Innovation Center, Moscow, Russia. alin.chernova@gmail.com., Gubaev RF; Skolkovo Institute of Science and Technology (Skoltech), Bolshoy Boulevard 30, bld. 1, Moscow, 121205, Russia.; LLC 'OIL GENE', Skolkovo Innovation Center, Moscow, Russia., Singh A; Department of Biological Sciences, University of Southern California, Los Angeles, CA, 90089, USA., Sherbina K; Department of Biological Sciences, University of Southern California, Los Angeles, CA, 90089, USA., Goryunova SV; Skolkovo Institute of Science and Technology (Skoltech), Bolshoy Boulevard 30, bld. 1, Moscow, 121205, Russia.; Vavilov Institute of General Genetics, Russian Academy of Sciences, Gubkin st. 3, Moscow, 119991, Russia.; FSBSI Lorch Potato Research Institute, Lorkha Str. 23, Kraskovo, 140051, Russia., Martynova EU; Skolkovo Institute of Science and Technology (Skoltech), Bolshoy Boulevard 30, bld. 1, Moscow, 121205, Russia., Goryunov DV; Skolkovo Institute of Science and Technology (Skoltech), Bolshoy Boulevard 30, bld. 1, Moscow, 121205, Russia.; MSU A.N. Belozersky Institute of Physico-Chemical Biology, Leninsky Gori 1, Building 40, Moscow, 119992, Russia., Boldyrev SV; Skolkovo Institute of Science and Technology (Skoltech), Bolshoy Boulevard 30, bld. 1, Moscow, 121205, Russia.; LLC 'OIL GENE', Skolkovo Innovation Center, Moscow, Russia., Vanyushkina AA; Skolkovo Institute of Science and Technology (Skoltech), Bolshoy Boulevard 30, bld. 1, Moscow, 121205, Russia., Anikanov NA; Skolkovo Institute of Science and Technology (Skoltech), Bolshoy Boulevard 30, bld. 1, Moscow, 121205, Russia., Stekolshchikova EA; Skolkovo Institute of Science and Technology (Skoltech), Bolshoy Boulevard 30, bld. 1, Moscow, 121205, Russia., Yushina EA; Skolkovo Institute of Science and Technology (Skoltech), Bolshoy Boulevard 30, bld. 1, Moscow, 121205, Russia.; FSBSI N P Bochkov Research Center of Medical Genetics, Moskvorechye St.1, Moscow, 115522, Russia., Demurin YN; Pustovoit All-Russia Research Institute of Oilseed Crops, Filatova St. 17, Krasnodar, 350038, Russia., Mukhina ZM; All-Russia Rice Research Institute, Krasnodar, 350921, Russia., Gavrilova VA; N. I. Vavilov Research Institute of Plant Genetic Resources (VIR), 42 B. Morskaja, St. Petersburg, 190000, Russia., Anisimova IN; N. I. Vavilov Research Institute of Plant Genetic Resources (VIR), 42 B. Morskaja, St. Petersburg, 190000, Russia., Karabitsina YI; N. I. Vavilov Research Institute of Plant Genetic Resources (VIR), 42 B. Morskaja, St. Petersburg, 190000, Russia., Alpatieva NV; N. I. Vavilov Research Institute of Plant Genetic Resources (VIR), 42 B. Morskaja, St. Petersburg, 190000, Russia., Chang PL; Department of Biological Sciences, University of Southern California, Los Angeles, CA, 90089, USA., Khaitovich P; Skolkovo Institute of Science and Technology (Skoltech), Bolshoy Boulevard 30, bld. 1, Moscow, 121205, Russia., Mazin PV; Skolkovo Institute of Science and Technology (Skoltech), Bolshoy Boulevard 30, bld. 1, Moscow, 121205, Russia., Nuzhdin SV; Department of Biological Sciences, University of Southern California, Los Angeles, CA, 90089, USA.
Jazyk: angličtina
Zdroj: BMC genomics [BMC Genomics] 2021 Jul 05; Vol. 22 (1), pp. 505. Date of Electronic Publication: 2021 Jul 05.
DOI: 10.1186/s12864-021-07768-y
Abstrakt: Background: Sunflower is an important oilseed crop domesticated in North America approximately 4000 years ago. During the last century, oil content in sunflower was under strong selection. Further improvement of oil properties achieved by modulating its fatty acid composition is one of the main directions in modern oilseed crop breeding.
Results: We searched for the genetic basis of fatty acid content variation by genotyping 601 inbred sunflower lines and assessing their lipid and fatty acid composition. Our genome-wide association analysis based on the genotypes for 15,483 SNPs and the concentrations of 23 fatty acids, including minor fatty acids, revealed significant genetic associations for eleven of them. Identified genomic regions included the loci involved in rare fatty acids variation on chromosomes 3 and 14, explaining up to 34.5% of the total variation of docosanoic acid (22:0) in sunflower oil.
Conclusions: This is the first large scale implementation of high-throughput lipidomic profiling to sunflower germplasm characterization. This study contributes to the genetic characterization of Russian sunflower collections, which made a substantial contribution to the development of sunflower as the oilseed crop worldwide, and provides new insights into the genetic control of oil composition that can be implemented in future studies.
Databáze: MEDLINE
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