Investigation into the bacterial diversity of sediment samples obtained from Berg River, Western Cape, South Africa.

Autor: Alegbeleye O; Department of Food Science, University of Campinas (UNICAMP), Rua Monteiro Lobato, 80, Campinas, SP, 13083-862, Brazil. seunalegbeleye@gmail.com., Alisoltani A; School of Medicine, University of California, Riverside, Riverside, CA, USA., Abia ALK; Antimicrobial Research Unit, College of Health Sciences, University of KwaZulu-Natal, Durban, South Africa., Awe AA; Department of Conservation and Marine Sciences, Faculty of Applied Sciences, Cape Peninsula University of Technology, Cape Town, South Africa., Adetunji AT; Department of Agriculture, Faculty of Applied Sciences, Cape Peninsula University of Technology, Western Cape, Wellington, 7654, South Africa., Rabiu S; Department of Environmental and Occupational Studies, Cape Peninsula University of Technology, Cape Town, South Africa., Opeolu BO; Extended Curriculum Programmes, Faculty of Applied Sciences, Cape Peninsula University of Technology, Cape Town, 8000, South Africa.
Jazyk: angličtina
Zdroj: Folia microbiologica [Folia Microbiol (Praha)] 2021 Dec; Vol. 66 (6), pp. 931-947. Date of Electronic Publication: 2021 Jul 05.
DOI: 10.1007/s12223-021-00893-x
Abstrakt: This study used conventional culturing and 16S rRNA metagenomics analyses to assess the diversity of bacterial communities in sediment samples obtained from the Berg River, Western Cape, South Africa. Samples were collected from six points: a residential and recreational area, an industrial area, an informal residential settlement, a point next to a wastewater treatment plant (WWTP), a pumping station, and a residential and agricultural farming area along the river. High bacterial counts recorded on general selective and differential culture media signify substantial microbial contamination along the sampling sites. The most prevalent bacterial phyla detected (through metagenomics analyses) along the sampling sites were Proteobacteria (61%), Planctomycetes (9.5%), Firmicutes (7.8%), Bacteroidetes (5%), Acidobacteria (4.6%), and Actinobacteria (4.6%). Some members of the identified predominant bacterial phyla, genera, and classes are important public health bacteria that have been implicated in human diseases and outbreaks, while some others are metal or hydrocarbon tolerant, indicating possible significant environmental pollution. Notable human pathogenic genera such as Bacillus, Clostridium, Shigella, Legionella, Mycobacterium, and Pseudomonas were identified in varying percentages at five of the six sampling areas. Fecal contamination was particularly rife at all residential areas, with the informal housing area being the most notably polluted. Diverse functional pathways were predicted for identified bacteria, such as those associated with different chronic and infectious human diseases as well as those related to hydrocarbon and metal remediation. The point next to a WWTP contained vastly diverse groups of bacterial contaminants as well as the most abundant pathway identities and titles.
(© 2021. Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i.)
Databáze: MEDLINE
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