Molecular Characterization and Survive Abilities of Salmonella Heidelberg Strains of Poultry Origin in Brazil.

Autor: Melo RT; Faculty of Veterinary Medicine, Federal University of Uberlândia, Uberlândia, Brazil., Galvão NN; Ministry of Agriculture, Livestock and Supply, Rio de Janeiro, Brazil., Guidotti-Takeuchi M; Faculty of Veterinary Medicine, Federal University of Uberlândia, Uberlândia, Brazil., Peres PABM; Faculty of Veterinary Medicine, Federal University of Uberlândia, Uberlândia, Brazil., Fonseca BB; Faculty of Veterinary Medicine, Federal University of Uberlândia, Uberlândia, Brazil., Profeta R; Department of Genetics, Ecology and Evolution (GEE), Federal University of Minas Gerais, Belo Horizonte, Brazil., Azevedo VAC; Department of Genetics, Ecology and Evolution (GEE), Federal University of Minas Gerais, Belo Horizonte, Brazil., Monteiro GP; Faculty of Veterinary Medicine, Federal University of Uberlândia, Uberlândia, Brazil., Brenig B; Institute of Veterinary Medicine, University of Göttingen, Göttingen, Germany., Rossi DA; Faculty of Veterinary Medicine, Federal University of Uberlândia, Uberlândia, Brazil.
Jazyk: angličtina
Zdroj: Frontiers in microbiology [Front Microbiol] 2021 Jun 18; Vol. 12, pp. 674147. Date of Electronic Publication: 2021 Jun 18 (Print Publication: 2021).
DOI: 10.3389/fmicb.2021.674147
Abstrakt: The aim of the study was to evaluate the genotypic and phenotypic characteristics of 20 strains of S. Heidelberg ( S H) isolated from broilers produced in southern Brazil. The similarity and presence of genetic determinants linked to virulence, antimicrobial resistance, biofilm formation, and in silico -predicted metabolic interactions revealed this serovar as a threat to public health. The presence of the ompC , invA , sodC , avrA , lpfA , and agfA genes was detected in 100% of the strains and the luxS gene in 70% of them. None of the strains carries the bla SHV , mcr-1, qnrA, qnrB , and qnrS genes. All strains showed a multidrug-resistant profile to at least three non-β-lactam drugs, which include colistin, sulfamethoxazole, and tetracycline. Resistance to penicillin, ceftriaxone (90%), meropenem (25%), and cefoxitin (25%) were associated with the presence of bla CTX-M and bla CMY-2 genes. Biofilm formation reached a mature stage at 25 and 37°C, especially with chicken juice (CJ) addition. The sodium hypochlorite 1% was the least efficient in controlling the sessile cells. Genomic analysis of two strains identified more than 100 virulence genes and the presence of resistance to 24 classes of antibiotics correlated to phenotypic tests. Protein-protein interaction (PPI) prediction shows two metabolic pathways correlation with biofilm formation. Virulence, resistance, and biofilm determinants must be constant monitoring in S H, due to the possibility of occurring infections extremely difficult to cure and due risk of the maintenance of the bacterium in production environments.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2021 Melo, Galvão, Guidotti-Takeuchi, Peres, Fonseca, Profeta, Azevedo, Monteiro, Brenig and Rossi.)
Databáze: MEDLINE
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