Evaluation of high salinity adaptation for lipid bio-accumulation in the green microalga Chlorella vulgaris .

Autor: Almutairi AW; Biological Sciences Department, Rabigh-Faculty of Science & Arts, King Abdulaziz University, Saudi Arabia., El-Sayed AEB; Algal Biotechnology Unit, National Research Centre, Egypt., Reda MM; Central Lab for Environmental Quality Monitoring, National Water Research Center, Egypt.
Jazyk: angličtina
Zdroj: Saudi journal of biological sciences [Saudi J Biol Sci] 2021 Jul; Vol. 28 (7), pp. 3981-3988. Date of Electronic Publication: 2021 Apr 20.
DOI: 10.1016/j.sjbs.2021.04.007
Abstrakt: Aiming at the reutilizing wastewater for algal growth and biomass production, a saline water rejected from reverse osmosis (RO) facility (salinity 67.59 g L -1 ) was used to cultivate the pre-adapted green microalga Chlorella vulgaris . The inoculum was prepared by growing cells in modified BG-11 medium, and adaptation was performed by applying a gradual increase in salinity (56.0 g L -1 NaCl and 125 ppm FeSO 4 ·7H 2 O) to the culture in 200 L photobioreactor. Experiments using the adapted alga were performed using original-rejected water (ORW) and treated rejected water (TRW) comparing with the recommended growth medium (BG-11). The initial salinity of ORW was chemically reduced to 39.1 g L -1 to obtain TRW. Vertical photobioreactors (15 L) was used for indoor growth experiments. Growth in BG-11 resulted in 1.23 g L -1 , while the next adaptation growth reached 2.14 g L -1 of dry biomass. The dry weights of re-cultivated Chlorella after adaptation were 1.49 and 2.19 g L -1 from ORW and TRW; respectively. The cellular oil content was only 12% when cells grown under control conditions verses to 14.3 and 15.42% with original and treated water, respectively. Induction of stress affected the fatty acid methyl esters (FAMEs) profile and the properties of the resulting biodiesel. The present results indicated that induction of stress by high salinity improves the quality of FAMEs that can be used as a promising biodiesel fuel.
Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(© 2021 The Author(s).)
Databáze: MEDLINE
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