Towards integrated production of an influenza A vaccine candidate with MDCK suspension cells.
Autor: | Bissinger T; Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany., Wu Y; Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany.; State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China., Marichal-Gallardo P; Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany., Riedel D; Facility for Transmission Electron Microscopy, Max Planck Institute for Biophysical Chemistry, Goettingen, Germany., Liu X; Shanghai BioEngine Sci-Tech Co., Shanghai, China., Genzel Y; Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany., Tan WS; State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.; Shanghai BioEngine Sci-Tech Co., Shanghai, China., Reichl U; Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany.; Chair of Bioprocess Engineering, Otto von Guericke University Magdeburg, Magdeburg, Germany. |
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Jazyk: | angličtina |
Zdroj: | Biotechnology and bioengineering [Biotechnol Bioeng] 2021 Oct; Vol. 118 (10), pp. 3996-4013. Date of Electronic Publication: 2021 Jul 20. |
DOI: | 10.1002/bit.27876 |
Abstrakt: | Seasonal influenza epidemics occur both in northern and southern hemispheres every year. Despite the differences in influenza virus surface antigens and virulence of seasonal subtypes, manufacturers are well-adapted to respond to this periodical vaccine demand. Due to decades of influenza virus research, the development of new influenza vaccines is relatively straight forward. In similarity with the ongoing coronavirus disease 2019 pandemic, vaccine manufacturing is a major bottleneck for a rapid supply of the billions of doses required worldwide. In particular, egg-based vaccine production would be difficult to schedule and shortages of other egg-based vaccines with high demands also have to be anticipated. Cell culture-based production systems enable the manufacturing of large amounts of vaccines within a short time frame and expand significantly our options to respond to pandemics and emerging viral diseases. In this study, we present an integrated process for the production of inactivated influenza A virus vaccines based on a Madin-Darby Canine Kidney (MDCK) suspension cell line cultivated in a chemically defined medium. Very high titers of 3.6 log (© 2021 The Authors. Biotechnology and Bioengineering published by Wiley Periodicals LLC.) |
Databáze: | MEDLINE |
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