Cineole-containing nanoemulsion: Development, stability, and antibacterial activity.

Autor: Lima TS; Graduate Program in Biosciences (PPGB), Federal University of the São Francisco Valley (UNIVASF), Petrolina, Pernambuco, 56304-205, Brazil., Silva MFS; Graduate Program in Biosciences (PPGB), Federal University of the São Francisco Valley (UNIVASF), Petrolina, Pernambuco, 56304-205, Brazil., Nunes XP; Graduate Program in Biosciences (PPGB), Federal University of the São Francisco Valley (UNIVASF), Petrolina, Pernambuco, 56304-205, Brazil; College of Pharmaceutical Sciences (CFARM), Federal University of the São Francisco Valley (UNIVASF), Petrolina, Pernambuco, 56304-205, Brazil., Colombo AV; Graduate Program in Biosciences (PPGB), Federal University of the São Francisco Valley (UNIVASF), Petrolina, Pernambuco, 56304-205, Brazil; College of Pharmaceutical Sciences (CFARM), Federal University of the São Francisco Valley (UNIVASF), Petrolina, Pernambuco, 56304-205, Brazil., Oliveira HP; Graduate Program in Materials Science (PPGCM), Federal University of the São Francisco Valley (UNIVASF), Juazeiro, Bahia, 48902-300, Brazil., Goto PL; IMRCP Laboratory, UMR 5623 CNRS, Paul Sabatier University, 31062, Toulouse, France., Blanzat M; IMRCP Laboratory, UMR 5623 CNRS, Paul Sabatier University, 31062, Toulouse, France., Piva HL; Department of Chemistry, Center of Nanotechnology and Tissue Engineering -Photobiology and Photomedicine Research Group, Faculty of Philosophy, Sciences and Letters of Ribeirão Preto (FFCLRP), University of São Paulo (USP), Ribeirão Preto, São Paulo, 14040-901, Brazil., Tedesco AC; Department of Chemistry, Center of Nanotechnology and Tissue Engineering -Photobiology and Photomedicine Research Group, Faculty of Philosophy, Sciences and Letters of Ribeirão Preto (FFCLRP), University of São Paulo (USP), Ribeirão Preto, São Paulo, 14040-901, Brazil., Siqueira-Moura MP; Graduate Program in Biosciences (PPGB), Federal University of the São Francisco Valley (UNIVASF), Petrolina, Pernambuco, 56304-205, Brazil; College of Pharmaceutical Sciences (CFARM), Federal University of the São Francisco Valley (UNIVASF), Petrolina, Pernambuco, 56304-205, Brazil. Electronic address: marigilson.moura@univasf.edu.br.
Jazyk: angličtina
Zdroj: Chemistry and physics of lipids [Chem Phys Lipids] 2021 Sep; Vol. 239, pp. 105113. Date of Electronic Publication: 2021 Jun 30.
DOI: 10.1016/j.chemphyslip.2021.105113
Abstrakt: 1,8-cineole is a monoterpene commonly used by the food, cosmetic, and pharmaceutical industries owing to its flavor and fragrances properties. In addition, this bioactive monoterpene has demonstrated bactericidal and fungicidal activities. However, such activities are limited due to its low aqueous solubility and stability. This study aimed to develop nanoemulsion containing cineole and assess its stability and antibacterial activity in this context. The spontaneous emulsification method was used to prepare nanoemulsion (NE) formulations (F1, F2, F3, F4, and F5). Following the development of NE formulations, we chose the F1 formulation that presented an average droplet size (in diameter) of about 100 nm with narrow size distribution (PdI <0.2) and negative zeta potential (∼ - 35 mV). According to the analytical centrifugation method with photometric detection, F1 and F5 formulations were considered the most stable NE with lower droplet migration velocities. In addition, F1 formulation showed high incorporation efficiency (> 80 %) and TEM analyses demonstrated nanosized oil droplets with irregular spherical shapes and without any aggregation tendency. Antibacterial activity assessment showed that F1 NE was able to enhance the cineole action against Staphylococcus aureus, Enterococcus faecalis, and Streptococcus pyogenes. Therefore, using a simple and reproducible method of low energy emulsification we designed a stable nanoemulsion containing 1,8-cineole with improved antibacterial activity against Gram-positive strains.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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