Alterations in Taenia crassiceps cysticerci cytoskeleton induced by nitazoxanide and flubendazole.

Autor: de Lima NF; Laboratory of studies of the host-parasite relationship, Tropical Pathology and Public Health Institute, Federal University of Goias. Brazil., Picanço GA; Laboratory of studies of the host-parasite relationship, Tropical Pathology and Public Health Institute, Federal University of Goias. Brazil., Valencia DGR; Laboratorio de Biologia del Citoesqueleto, Departamento de Microbiologia y Parasitologia, Facultad de Medicina, Universidad Nacional Autonoma de Mexico (UNAM), México, DF 04510, Mexico., Villegas EOL; Laboratorio Central de Microscopía, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional (IPN), DF 11340, México., Mellado MDRE; Laboratorio Central de Microscopía, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional (IPN), DF 11340, México., Ambrosio JR; Laboratorio de Biologia del Citoesqueleto, Departamento de Microbiologia y Parasitologia, Facultad de Medicina, Universidad Nacional Autonoma de Mexico (UNAM), México, DF 04510, Mexico., Vinaud MC; Laboratory of studies of the host-parasite relationship, Tropical Pathology and Public Health Institute, Federal University of Goias. Brazil. Electronic address: mvinaud@ufg.br.
Jazyk: angličtina
Zdroj: Acta tropica [Acta Trop] 2021 Sep; Vol. 221, pp. 106027. Date of Electronic Publication: 2021 Jul 01.
DOI: 10.1016/j.actatropica.2021.106027
Abstrakt: Cysticercosis is the presence of Taenia solium larval stage in tissues such as central nervous system, skin, muscles and eye globe. The current treatment is based on albendazole and praziquantel which already present resistance reports. Therefore, the search for alternative treatments is paramount. The aim of this study was to determine the effect of flubendazole and nitazoxanide on cytoskeleton proteins from Taenia crassiceps cysticerci, an experimental model for cysticercosis. Cysticerci were cultured in RPMI supplemented medium containing nitazoxanide and/or flubendazole. 24 h after the exposure the cysticerci were processed for scanning and transmission electron microscopy and for protein analysis of the cytoskeleton. The proteins were detected through 1D electrophoresis and identified through Western Blot. Nitazoxanide exposure increased tubulin and actin quantifications in T. crassiceps cysticerci. While flubendazole alone and the drugs combinations induced an increase in α-tubulin and actin and decreased β-tubulin quantifications in the parasite. Morphological changes such as swelling and rupture of vesicle, stiff membrane, decrease in movements were observed when the cysticerci were incubated with the different compounds. In conclusion the drugs induced significative impact in the parasite`s cytoskeleton and may be considered as alternative treatments for cysticercosis.
(Copyright © 2021 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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