Fundamental limits on the rate of bacterial growth and their influence on proteomic composition.

Autor:	Belliveau NM; Department of Biology, Howard Hughes Medical Institute, University of Washington, Seattle, WA 98105, USA., Chure G; Department of Applied Physics, California Institute of Technology, Pasadena, CA 91125, USA., Hueschen CL; Department of Chemical Engineering, Stanford University, Stanford, CA 94305, USA., Garcia HG; Department of Molecular Cell Biology and Department of Physics, University of California Berkeley, Berkeley, CA 94720, USA., Kondev J; Department of Physics, Brandeis University, Waltham, MA 02453, USA., Fisher DS; Department of Applied Physics, Stanford University, Stanford, CA 94305, USA., Theriot JA; Department of Biology, Howard Hughes Medical Institute, University of Washington, Seattle, WA 98105, USA. Electronic address: jtheriot@uw.edu., Phillips R; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA; Department of Physics, California Institute of Technology, Pasadena, CA 91125, USA. Electronic address: phillips@pboc.caltech.edu.
Jazyk:	angličtina
Zdroj:	Cell systems [Cell Syst] 2021 Sep 22; Vol. 12 (9), pp. 924-944.e2. Date of Electronic Publication: 2021 Jul 01.
DOI:	10.1016/j.cels.2021.06.002
Abstrakt:	Despite abundant measurements of bacterial growth rate, cell size, and protein content, we lack a rigorous understanding of what sets the scale of these quantities and when protein abundances should (or should not) depend on growth rate. Here, we estimate the basic requirements and physical constraints on steady-state growth by considering key processes in cellular physiology across a collection of Escherichia coli proteomic data covering ≈4,000 proteins and 36 growth rates. Our analysis suggests that cells are predominantly tuned for the task of cell doubling across a continuum of growth rates; specific processes do not limit growth rate or dictate cell size. We present a model of proteomic regulation as a function of nutrient supply that reconciles observed interdependences between protein synthesis, cell size, and growth rate and propose that a theoretical inability to parallelize ribosomal synthesis places a firm limit on the achievable growth rate. A record of this paper's transparent peer review process is included in the supplemental information. Competing Interests: Declaration of interests J.A.T. is chief scientific advisor at the Allen Institute for Cell Science (Seattle, WA, 98109). The authors otherwise declare no competing interests. (Copyright © 2021 Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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