| Autor: |
Santos TS; Post-Graduating Program in Health and Environment, Tiradentes University, Av. Murilo Dantas, 300, Aracaju Sergipe 49010-390, Brazil., Santos IDDD; Post-Graduating Program in Health and Environment, Tiradentes University, Av. Murilo Dantas, 300, Aracaju Sergipe 49010-390, Brazil., Pereira-Filho RN; Institute of Technology and Research (ITP), Av. Murilo Dantas, 300, Aracaju 49010-390, Brazil., Gomes SVF; Post-Graduating Program in Health and Environment, Tiradentes University, Av. Murilo Dantas, 300, Aracaju Sergipe 49010-390, Brazil., Lima-Verde IB; Post-Graduating Program in Health and Environment, Tiradentes University, Av. Murilo Dantas, 300, Aracaju Sergipe 49010-390, Brazil.; Institute of Technology and Research (ITP), Av. Murilo Dantas, 300, Aracaju 49010-390, Brazil., Marques MN; Post-Graduating Program in Health and Environment, Tiradentes University, Av. Murilo Dantas, 300, Aracaju Sergipe 49010-390, Brazil.; Institute of Technology and Research (ITP), Av. Murilo Dantas, 300, Aracaju 49010-390, Brazil., Cardoso JC; Post-Graduating Program in Health and Environment, Tiradentes University, Av. Murilo Dantas, 300, Aracaju Sergipe 49010-390, Brazil.; Institute of Technology and Research (ITP), Av. Murilo Dantas, 300, Aracaju 49010-390, Brazil., Severino P; Institute of Technology and Research (ITP), Av. Murilo Dantas, 300, Aracaju 49010-390, Brazil.; Tiradentes Institute, 150 Mt Vernon St, Dorchester, MA 02125, USA., Souto EB; CEB-Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.; Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Coimbra, Pólo das Ciências da Saúde, Azinhaga de Santa Comba, 3000-548 Coimbra, Portugal., Albuquerque-Júnior RLC; Post-Graduating Program in Health and Environment, Tiradentes University, Av. Murilo Dantas, 300, Aracaju Sergipe 49010-390, Brazil.; Institute of Technology and Research (ITP), Av. Murilo Dantas, 300, Aracaju 49010-390, Brazil. |
| Abstrakt: |
Plant extracts rich in phenolic compounds have been demonstrated to accelerate wound healing, but their use by oral route has been poorly studied. The leaves of Vitis labrusca are rich in phenolic acids and flavonoids. The goal of this study was to assess the healing properties of the oral administration of hydroalcoholic extract of V. labrusca leaves (HEVL) in a murine model. HEVL was obtained by Soxhlet and dynamic maceration, and their yield and phenolic acids and flavonoid contents were determined. For the wound healing assay, 8 mm wounds were performed on the back of 48 Wistar rats, assigned into four groups ( n = 12): CTR (distilled water), HEVL100, HEVL200, and HEVL300 (HEVL at 100, 200, and 300 mg/kg, respectively). On days 7 and 14, wound closure rates were assessed, and the healing wounds were subjected to histological analysis. Soxhlet-obtained extract was selected for the wound healing assay because it provided a higher yield and phenolic acid and flavonoid contents. HEVL significantly reduced leukocytosis in the peripheral blood ( p < 0.05), accelerated wound closure ( p < 0.05), and improved collagenization ( p < 0.05) on day 7, as well as enhanced the epidermal tissue thickness ( p < 0.001) and elastic fiber deposition on day 14 ( p < 0.01). Furthermore, HEVL promoted an increase in the histological grading of wound healing on both days 7 and 14 ( p < 0.01). The doses of 200 and 300 mg/kg provided better results than 100 mg/Kg. Our data provide histological evidence that the oral administration of HEVL improves wound healing in rodents. Therefore, the extract can be a potential oral medicine for healing purposes. |
