[Partial purification of tissue thromboplastin by the gel filtration method and its use for reproducing the generalized decompensated thrombinogenesis syndrome].

Autor: Magerovskiĭ IuV, Monastyrskiĭ VA, Gaĭda AV, Birka II, Voroniak MI
Jazyk: ruština
Zdroj: Voprosy meditsinskoi khimii [Vopr Med Khim] 1988 May-Jun; Vol. 34 (3), pp. 34-9.
Abstrakt: Tissue thromboplastin was purified using various chromatographic resins: Sephadex G-200, Ultragel AcA 34, Acrylex P-300 and Toyopearl HW-55F. The purity of the preparations obtained was evaluated by means of the ratio of the thromboplastin solutions absorbency at 540 nm and a protein concentration in the solution expressed as mg/ml. The most purified preparations of tissue thromboplastin were obtained after gel filtration on Toyopearl HW-55F. The thromboplastin preparation produced was used for experimental reproduction of the syndrome of generalized decompensated thrombinogenesis.
Databáze: MEDLINE