| Autor: |
Janovits PM; Laboratório de Farmacologia, Instituto Butantan, São Paulo 05503-900, Brazil.; Centre of Excellence in New Target Discovery (CENTD), Instituto Butantan, São Paulo 05503-900, Brazil., Leiguez E; Laboratório de Farmacologia, Instituto Butantan, São Paulo 05503-900, Brazil.; Centre of Excellence in New Target Discovery (CENTD), Instituto Butantan, São Paulo 05503-900, Brazil., Portas V; Centre of Excellence in New Target Discovery (CENTD), Instituto Butantan, São Paulo 05503-900, Brazil.; Laboratório de Desenvolvimento e Inovação, Instituto Butantan, São Paulo 05503-900, Brazil., Teixeira C; Laboratório de Farmacologia, Instituto Butantan, São Paulo 05503-900, Brazil.; Centre of Excellence in New Target Discovery (CENTD), Instituto Butantan, São Paulo 05503-900, Brazil. |
| Abstrakt: |
Matrix metalloproteinases (MMPs) are proteolytic enzymes that have been associated with the pathogenesis of inflammatory diseases and obesity. Adipose tissue in turn is an active endocrine organ capable of secreting a range of proinflammatory mediators with autocrine and paracrine properties, which contribute to the inflammation of adipose tissue and adjacent tissues. However, the potential inflammatory effects of MMPs in adipose tissue cells are still unknown. This study investigates the effects of BmooMPα-I, a single-domain snake venom metalloproteinase (SVMP), in activating an inflammatory response by 3T3-L1 preadipocytes in culture, focusing on prostaglandins (PGs), cytokines, and adipocytokines biosynthesis and mechanisms involved in prostaglandin E 2 (PGE 2 ) release. The results show that BmooMPα-I induced the release of PGE 2 , prostaglandin I 2 (PGI 2 ), monocyte chemoattractant protein-1 (MCP-1), and adiponectin by preadipocytes. BmooMPα-I-induced PGE 2 biosynthesis was dependent on group-IIA-secreted phospholipase A 2 (sPLA 2 -IIA), cytosolic phospholipase A 2 -α (cPLA 2 -α), and cyclooxygenase (COX)-1 and -2 pathways. Moreover, BmooMPα-I upregulated COX-2 protein expression but not microsomal prostaglandin E synthase-1 (mPGES-1) expression. In addition, we demonstrate that the enzymatic activity of BmooMPα-I is essential for the activation of prostanoid synthesis pathways in preadipocytes. These data highlight preadipocytes as important targets for metalloproteinases and provide new insights into the contribution of these enzymes to the inflammation of adipose tissue and tissues adjacent to it. |
