| Autor: |
Kumar U; Department of Biochemistry and Genetics, Barkatullah University, Bhopal 462026, India., Khandia R; Department of Biochemistry and Genetics, Barkatullah University, Bhopal 462026, India., Singhal S; Department of Biochemistry and Genetics, Barkatullah University, Bhopal 462026, India., Puranik N; Department of Biochemistry and Genetics, Barkatullah University, Bhopal 462026, India., Tripathi M; ICAR-National Institute of High Security Animal Diseases, Bhopal 462043, India., Pateriya AK; ICAR-National Institute of High Security Animal Diseases, Bhopal 462043, India., Khan R; Microfluidics & MEMS Center, (MRS & CFC), CSIR-Advanced Materials and Processes Research Institute (AMPRI), Hoshangabad Road, Bhopal 462026, India., Emran TB; Department of Pharmacy, BGC Trust University Bangladesh, Chittagong 4381, Bangladesh., Dhama K; Division of Pathology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, India., Munjal A; Department of Biochemistry and Genetics, Barkatullah University, Bhopal 462026, India., Alqahtani T; Department of Pharmacology, College of Pharmacy, King Khalid University, Abha 62529, Saudi Arabia., Alqahtani AM; Department of Pharmacology, College of Pharmacy, King Khalid University, Abha 62529, Saudi Arabia. |
| Abstrakt: |
Uneven codon usage within genes as well as among genomes is a usual phenomenon across organisms. It plays a significant role in the translational efficiency and evolution of a particular gene. EPB41L3 is a tumor suppressor protein-coding gene, and in the present study, the pattern of codon usage was envisaged. The full-length sequences of the EPB41L3 gene for the human, brown rat, domesticated cattle, and Sumatran orangutan available at the NCBI were retrieved and utilized to analyze CUB patterns across the selected mammalian species. Compositional properties, dinucleotide abundance, and parity analysis showed the dominance of A and G whilst RSCU analysis indicated the dominance of G/C-ending codons. The neutrality plot plotted between GC12 and GC3 to determine the variation between the mutation pressure and natural selection indicated the dominance of selection pressure (R = 0.926; p < 0.00001) over the three codon positions across the gene. The result is in concordance with the codon adaptation index analysis and the ENc-GC3 plot analysis, as well as the translational selection index (P2). Overall selection pressure is the dominant pressure acting during the evolution of the EPB41L3 gene. |
