| Autor: |
Siniagina MN; Institute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, Russia., Markelova MI; Institute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, Russia., Boulygina EA; Institute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, Russia., Laikov AV; Institute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, Russia., Khusnutdinova DR; Institute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, Russia., Abdulkhakov SR; Institute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, Russia.; Department of Outpatient Therapy and General Medical Practice, Kazan State Medical University, 420012 Kazan, Russia., Danilova NA; Department of Gastroenterology, Republican Clinical Hospital of Tatarstan Republic, 420064 Kazan, Russia., Odintsova AH; Department of Gastroenterology, Republican Clinical Hospital of Tatarstan Republic, 420064 Kazan, Russia., Abdulkhakov RA; Department of Hospital Medicine, Kazan State Medical University, 420012 Kazan, Russia., Grigoryeva TV; Institute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, Russia. |
| Abstrakt: |
Crohn's disease (CD) is characterized by a chronic, progressive inflammation across the gastrointestinal tract with a series of exacerbations and remissions. A significant factor in the CD pathogenesis is an imbalance in gut microbiota composition, particularly the prevalence of Escherichia coli . In the present study, the genomes of sixty-three E. coli strains from the gut of patients with CD and healthy subjects were sequenced. In addition, eighteen E. coli -like metagenome-assembled genomes (MAGs) were reconstructed from the shotgun-metagenome sequencing data of fecal samples. The comparative analysis revealed the similarity of E. coli genomes regardless of the origin of the strain. The strains exhibited similar genetic patterns of virulence, antibiotic resistance, and bacteriocin-producing systems. The study showed antagonistic activity of E. coli strains and the metabolic features needed for their successful competition in the human gut environment. These observations suggest complex bacterial interactions within the gut which may affect the host and cause intestinal damage. |
