Differential Expression of BOC , SPOCK2 , and GJD3 Is Associated with Brain Metastasis of ER-Negative Breast Cancers.

Autor: Pedrosa RMSM; Department of Pathology, Erasmus University Medical Center, Wytemaweg 80, 3000 DR Rotterdam, The Netherlands.; The Tumor Immuno-Pathology (TIP) Laboratory, Erasmus University Medical Center, Wytemaweg 80, 3000 DR Rotterdam, The Netherlands., Wismans LV; The Tumor Immuno-Pathology (TIP) Laboratory, Erasmus University Medical Center, Wytemaweg 80, 3000 DR Rotterdam, The Netherlands.; Department of Surgery, Erasmus University Medical Center, Wytemaweg 80, 3000 DR Rotterdam, The Netherlands., Sinke R; Laboratory of Pathology, Pathan BV, Kleiweg 500, 3045 PM Rotterdam, The Netherlands., van der Weiden M; Department of Pathology, Erasmus University Medical Center, Wytemaweg 80, 3000 DR Rotterdam, The Netherlands., van Eijck CHJ; The Tumor Immuno-Pathology (TIP) Laboratory, Erasmus University Medical Center, Wytemaweg 80, 3000 DR Rotterdam, The Netherlands.; Department of Surgery, Erasmus University Medical Center, Wytemaweg 80, 3000 DR Rotterdam, The Netherlands., Kros JM; Department of Pathology, Erasmus University Medical Center, Wytemaweg 80, 3000 DR Rotterdam, The Netherlands.; The Tumor Immuno-Pathology (TIP) Laboratory, Erasmus University Medical Center, Wytemaweg 80, 3000 DR Rotterdam, The Netherlands.; Brain Tumor Center, Erasmus Medical Center, Wytemaweg 80, 3000 DR Rotterdam, The Netherlands., Mustafa DAM; Department of Pathology, Erasmus University Medical Center, Wytemaweg 80, 3000 DR Rotterdam, The Netherlands.; The Tumor Immuno-Pathology (TIP) Laboratory, Erasmus University Medical Center, Wytemaweg 80, 3000 DR Rotterdam, The Netherlands.
Jazyk: angličtina
Zdroj: Cancers [Cancers (Basel)] 2021 Jun 15; Vol. 13 (12). Date of Electronic Publication: 2021 Jun 15.
DOI: 10.3390/cancers13122982
Abstrakt: Background: Brain metastasis is considered one of the major causes of mortality in breast cancer patients. To invade the brain, tumor cells need to pass the blood-brain barrier by mechanisms that are partially understood. In primary ER-negative breast cancers that developed brain metastases, we found that some of the differentially expressed genes play roles in the T cell response. The present study aimed to identify genes involved in the formation of brain metastasis independently from the T cell response.
Method: Previously profiled primary breast cancer samples were reanalyzed. Genes that were found to be differentially expressed were confirmed by RT-PCR and by immunohistochemistry using an independent cohort of samples.
Results: BOC, SPOCK2, and GJD3 were overexpressed in the primary breast tumors that developed brain metastasis. BOC expression was successfully validated at the protein level. SPOCK2 was validated at both mRNA and protein levels. SPOCK2 and GJD3 mRNA overexpression were also found to be associated with cerebral metastasis in an external online database consisting of 204 primary breast cancers.
Conclusion: The overexpression of BOC, SPOCK2 , and GJD3 is associated with the invasion of breast cancer into the brain. Further studies to determine their specific function and potential value as brain metastasis biomarkers are required.
Databáze: MEDLINE
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