Biosynthesis of Bacterial Cellulose by Extended Cultivation with Multiple Removal of BC Pellicles.

Autor: Skiba EA; Institute for Problems of Chemical and Energetic Technologies, Siberian Branch of the Russian Academy of Sciences (IPCET SB RAS), 659322 Biysk, Altai Krai, Russia., Shavyrkina NA; Institute for Problems of Chemical and Energetic Technologies, Siberian Branch of the Russian Academy of Sciences (IPCET SB RAS), 659322 Biysk, Altai Krai, Russia.; Biysk Technological Institute, Polzunov Altai State Technical University, 659305 Biysk, Altai Krai, Russia., Budaeva VV; Institute for Problems of Chemical and Energetic Technologies, Siberian Branch of the Russian Academy of Sciences (IPCET SB RAS), 659322 Biysk, Altai Krai, Russia., Sitnikova AE; Institute for Problems of Chemical and Energetic Technologies, Siberian Branch of the Russian Academy of Sciences (IPCET SB RAS), 659322 Biysk, Altai Krai, Russia.; Biysk Technological Institute, Polzunov Altai State Technical University, 659305 Biysk, Altai Krai, Russia., Korchagina AA; Institute for Problems of Chemical and Energetic Technologies, Siberian Branch of the Russian Academy of Sciences (IPCET SB RAS), 659322 Biysk, Altai Krai, Russia., Bychin NV; Institute for Problems of Chemical and Energetic Technologies, Siberian Branch of the Russian Academy of Sciences (IPCET SB RAS), 659322 Biysk, Altai Krai, Russia., Gladysheva EK; Institute for Problems of Chemical and Energetic Technologies, Siberian Branch of the Russian Academy of Sciences (IPCET SB RAS), 659322 Biysk, Altai Krai, Russia., Pavlov IN; Institute for Problems of Chemical and Energetic Technologies, Siberian Branch of the Russian Academy of Sciences (IPCET SB RAS), 659322 Biysk, Altai Krai, Russia.; Biysk Technological Institute, Polzunov Altai State Technical University, 659305 Biysk, Altai Krai, Russia., Zharikov AN; Chair of Neymark Departmental Surgery and Hospital Surgery, Altai State Medical University, 656038 Barnaul, Altai Krai, Russia., Lubyansky VG; Chair of Neymark Departmental Surgery and Hospital Surgery, Altai State Medical University, 656038 Barnaul, Altai Krai, Russia., Semyonova EN; Anatomic Pathology Department, Altai Krai Clinical Hospital, 656024 Barnaul, Altai Krai, Russia., Sakovich GV; Institute for Problems of Chemical and Energetic Technologies, Siberian Branch of the Russian Academy of Sciences (IPCET SB RAS), 659322 Biysk, Altai Krai, Russia.
Jazyk: angličtina
Zdroj: Polymers [Polymers (Basel)] 2021 Jun 28; Vol. 13 (13). Date of Electronic Publication: 2021 Jun 28.
DOI: 10.3390/polym13132118
Abstrakt: Extended cultivation with multiple removal of BC pellicles is proposed herein as a new biosynthetic process for bacterial cellulose (BC). This method enhances the BC surface area by 5-11 times per unit volume of the growth medium, improving the economic efficiency of biosynthesis. The resultant BC gel-films were thin, transparent, and congruent. The degree of polymerization (DP) and elastic modulus (EM) depended on the number of BC pellicle removals, vessel shape, and volume. The quality of BC from removals II-III to VII was better than from removal I. The process scale-up of 1:40 by volume increased DP by 1.5 times and EM by 5 times. A fact was established that the symbiotic Medusomyces gisevii Sa-12 was adaptable to exhausted growth medium: the medium was able to biosynthesize BC for 60 days, while glucose ran low at 24 days. On extended cultivation, DP and EM were found to decline by 39-64% and 57-65%, respectively. The BC gel-films obtained upon removals I-VI were successfully trialed in experimental tension-free hernioplasty.
Databáze: MEDLINE
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