Autor: |
Bourgou S; Laboratory of Medicinal and Aromatics Plant, Biotechnology Center of Borj-Cedria, BP 901, 2050 Hammam-Lif, Tunisia., Bettaieb Rebey I; Laboratory of Medicinal and Aromatics Plant, Biotechnology Center of Borj-Cedria, BP 901, 2050 Hammam-Lif, Tunisia.; Laboratory of Chemistry of Natural Molecules, Gembloux Agro-Bio Tech, Université de Liège, Passage des Déportés 2, 5030 Gembloux, Belgium., Ben Kaab S; Laboratory of Medicinal and Aromatics Plant, Biotechnology Center of Borj-Cedria, BP 901, 2050 Hammam-Lif, Tunisia.; Integrated and Urban Plant Pathology Laboratory, Gembloux Agro-Bio Tech, University of Liège 2, 8 Passage des Déportés 2, 5030 Gembloux, Belgium., Hammami M; Laboratory of Medicinal and Aromatics Plant, Biotechnology Center of Borj-Cedria, BP 901, 2050 Hammam-Lif, Tunisia., Dakhlaoui S; Laboratory of Medicinal and Aromatics Plant, Biotechnology Center of Borj-Cedria, BP 901, 2050 Hammam-Lif, Tunisia., Sawsen S; Laboratory of Medicinal and Aromatics Plant, Biotechnology Center of Borj-Cedria, BP 901, 2050 Hammam-Lif, Tunisia., Msaada K; Laboratory of Medicinal and Aromatics Plant, Biotechnology Center of Borj-Cedria, BP 901, 2050 Hammam-Lif, Tunisia., Isoda H; Faculty of Life and Environmental Sciences, School of Integrative and Global Majors (SIGMA), and Alliance for Research on Mediterranean and North Africa (ARENA), University of Tsukuba, Tsukuba 3058572, Japan., Ksouri R; Laboratory of Medicinal and Aromatics Plant, Biotechnology Center of Borj-Cedria, BP 901, 2050 Hammam-Lif, Tunisia., Fauconnier ML; Laboratory of Chemistry of Natural Molecules, Gembloux Agro-Bio Tech, Université de Liège, Passage des Déportés 2, 5030 Gembloux, Belgium. |
Abstrakt: |
A comparative study of bioactive lipids extraction from black cumin ( Nigella sativa L.) and basil ( Ocimum basilicum L.) seeds using conventional petroleum-based solvent and green solvent 2-methyltetrahydrofuran (MeTHF) was performed. MeTHF extraction allowed obtaining the highest oil yield in black cumin (34%). Regarding fatty acids composition, linoleic acid (61%) and α-linolenic (78%) were relevant in black cumin and basil green and conventionally extracted oils, respectively. Besides, MeTHF allowed obtaining higher tocopherols and total phenolics contents in black cumin (400 mg/kg of oil and 12 mg EGA/g oil) and basil (317 mg/kg oil and 5 mg EGA/g oil) compared to hexane-extracted ones. The content of major phenolic compounds in the two seed oils, trans -hydroxycinnamic acid, rosmarinic acid, and thymol was enhanced by MeTHF extraction. Furthermore, MeTHF-extracted oils possess stronger antioxidant activities (radical scavenging, total antioxidant, and β-carotene bleaching activities) and high and similar anti-inflammatory capacity to hexane-extracted oils. In conclusion, the results revealed that MeTHF is efficient to replace hazardous solvents to extract oil from black cumin and basil seeds rich in compounds relevant to the human diet, including essential polyunsaturated fatty acids (n-6 and n-3), tocopherols, and phenolic compounds with improved biological activities. |