A New Water-Soluble Thermosensitive Star-Like Copolymer as a Promising Carrier of the Chemotherapeutic Drug Doxorubicin.

Autor: Chernykh M; Taras Shevchenko National University of Kyiv, Volodymyrska Street, 64, 01601 Kyiv, Ukraine., Zavalny D; Taras Shevchenko National University of Kyiv, Volodymyrska Street, 64, 01601 Kyiv, Ukraine., Sokolova V; Center for Nanointegration Duisburg-Essen (CeNIDE), Institute of Inorganic Chemistry, University of Duisburg-Essen, University Street, 5-7, 45117 Essen, Germany., Ponomarenko S; Taras Shevchenko National University of Kyiv, Volodymyrska Street, 64, 01601 Kyiv, Ukraine., Prylutska S; Department of Physiology, Plant Biochemistry and Bioenergetics, National University of Life and Environmental Science of Ukraine, Heroiv Oborony Street, 15, 03041 Kyiv, Ukraine., Kuziv Y; Taras Shevchenko National University of Kyiv, Volodymyrska Street, 64, 01601 Kyiv, Ukraine., Chumachenko V; Taras Shevchenko National University of Kyiv, Volodymyrska Street, 64, 01601 Kyiv, Ukraine., Marynin A; National University of Food Technologies of Ukraine, Volodymyrska Street, 01033 Kyiv, Ukraine., Kutsevol N; Taras Shevchenko National University of Kyiv, Volodymyrska Street, 64, 01601 Kyiv, Ukraine., Epple M; Center for Nanointegration Duisburg-Essen (CeNIDE), Institute of Inorganic Chemistry, University of Duisburg-Essen, University Street, 5-7, 45117 Essen, Germany., Ritter U; Institute of Chemistry and Biotechnology, Technical University of Ilmenau, Weimarer Street, 25, 98693 Ilmenau, Germany., Piosik J; Laboratory of Biophysics, Intercollegiate Faculty of Biotechnology UG-MUG, University of Gdansk, Abrahama 58, 80-307 Gdańsk, Poland., Prylutskyy Y; Taras Shevchenko National University of Kyiv, Volodymyrska Street, 64, 01601 Kyiv, Ukraine.
Jazyk: angličtina
Zdroj: Materials (Basel, Switzerland) [Materials (Basel)] 2021 Jun 24; Vol. 14 (13). Date of Electronic Publication: 2021 Jun 24.
DOI: 10.3390/ma14133517
Abstrakt: A new water-soluble thermosensitive star-like copolymer, dextran-graft-poly-N-iso-propilacrylamide (D-g-PNIPAM), was created and characterized by various techniques (size-exclusion chromatography, differential scanning calorimetry, Fourier-transform infrared (FTIR) spectroscopy, and dynamic light scattering (DLS) spectroscopy). The viability of cancer cell lines (human transformed cervix epithelial cells, HeLa) as a model for cancer cells was studied using MTT and Live/Dead assays after incubation with a D-g-PNIPAM copolymer as a carrier for the drug doxorubicin (Dox) as well as a D-g-PNIPAM + Dox mixture as a function of the concentration. FTIR spectroscopy clearly indicated the complex formation of Dox with the D-g-PNIPAM copolymer. The size distribution of particles in Hank's solution was determined by the DLS technique at different temperatures. The in vitro uptake of the studied D-g-PNIPAM + Dox nanoparticles into cancer cells was demonstrated by confocal laser scanning microscopy. It was found that D-g-PNIPAM + Dox nanoparticles in contrast to Dox alone showed higher toxicity toward cancer cells. All of the aforementioned facts indicate a possibility of further preclinical studies of the water-soluble D-g-PNIPAM particles' behavior in animal tumor models in vivo as promising carriers of anticancer agents.
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje