| Autor: |
Orłowska R; Department of Plant Physiology and Biochemistry, Plant Breeding and Acclimatization Institute-National Research Institute, Radzików, 05-870 Błonie, Poland., Pachota KA; Department of Plant Physiology and Biochemistry, Plant Breeding and Acclimatization Institute-National Research Institute, Radzików, 05-870 Błonie, Poland., Dynkowska WM; Department of Plant Physiology and Biochemistry, Plant Breeding and Acclimatization Institute-National Research Institute, Radzików, 05-870 Błonie, Poland., Niedziela A; Department of Plant Physiology and Biochemistry, Plant Breeding and Acclimatization Institute-National Research Institute, Radzików, 05-870 Błonie, Poland., Bednarek PT; Department of Plant Physiology and Biochemistry, Plant Breeding and Acclimatization Institute-National Research Institute, Radzików, 05-870 Błonie, Poland. |
| Jazyk: |
angličtina |
| Zdroj: |
International journal of molecular sciences [Int J Mol Sci] 2021 Jun 24; Vol. 22 (13). Date of Electronic Publication: 2021 Jun 24. |
| DOI: |
10.3390/ijms22136783 |
| Abstrakt: |
A plant genome usually encompasses different families of transposable elements (TEs) that may constitute up to 85% of nuclear DNA. Under stressful conditions, some of them may activate, leading to sequence variation. In vitro plant regeneration may induce either phenotypic or genetic and epigenetic changes. While DNA methylation alternations might be related, i.e., to the Yang cycle problems, DNA pattern changes, especially DNA demethylation, may activate TEs that could result in point mutations in DNA sequence changes. Thus, TEs have the highest input into sequence variation (SV). A set of barley regenerants were derived via in vitro anther culture. High Performance Liquid Chromatography (RP-HPLC), used to study the global DNA methylation of donor plants and their regenerants, showed that the level of DNA methylation increased in regenerants by 1.45% compared to the donors. The Methyl-Sensitive Transposon Display (MSTD) based on methylation-sensitive Amplified Fragment Length Polymorphism (metAFLP) approach demonstrated that, depending on the selected elements belonging to the TEs family analyzed, varying levels of sequence variation were evaluated. DNA sequence contexts may have a different impact on SV generated by distinct mobile elements belonged to various TE families. Based on the presented study, some of the selected mobile elements contribute differently to TE-related SV. The surrounding context of the TEs DNA sequence is possibly important here, and the study explained some part of SV related to those contexts. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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