| Autor: |
Ogneva ZV; Laboratory of Biotechnology, Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, Russia., Volkonskaia VV; Laboratory of Biotechnology, Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, Russia.; The School of Natural Sciences, Far Eastern Federal University, 690090 Vladivostok, Russia., Dubrovina AS; Laboratory of Biotechnology, Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, Russia., Suprun AR; Laboratory of Biotechnology, Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, Russia., Aleynova OA; Laboratory of Biotechnology, Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, Russia., Kiselev KV; Laboratory of Biotechnology, Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, Russia. |
| Abstrakt: |
Excessive ultraviolet B (UV-B) irradiation is one of the most serious threats leading to severe crop production losses. It is known that secondary metabolite biosynthesis plays an important role in plant defense and forms a protective shield against excessive UV-B irradiation. The contents of stilbenes and other plant phenolics are known to sharply increase after UV-B irradiation, but there is little direct evidence for the involvement of stilbenes and other plant phenolics in plant UV-B protection. This study showed that foliar application of trans -resveratrol (1 and 5 mM) and trans -piceid (5 mM) considerably increased tolerance to a shock of UV-B (10 min at 1800 µW cm -2 of irradiation intensity) of four-week-old Arabidopsis thaliana plants that are naturally incapable of stilbene production. Application of trans -resveratrol and trans -piceid increased the leaf survival rates by 1-2%. This stilbene-induced improvement in UV-B tolerance was higher than after foliar application of the stilbene precursors, p- coumaric and trans -cinnamic acids (only 1-3%), but less than that after treatment with octocrylene (19-24%), a widely used UV-B absorber. Plant treatment with trans -resveratrol increased expression of antioxidant and stress-inducible genes in A. thaliana plants and decreased expression of DNA repair genes. This study directly demonstrates an important positive role of stilbenes in plant tolerance to excessive UV-B irradiation, and offers a new approach for plant UV-B protection. |
