| Autor: |
Tlak Gajger I; Department for Biology and Pathology of Fish and Bees, Faculty of Veterinary Medicine, University of Zagreb, 10000 Zagreb, Croatia., Šimenc L; Virology Unit, Institute of Microbiology and Parasitology, Veterinary Faculty, University of Ljubljana, Gerbičeva 60, 1000 Ljubljana, Slovenia., Toplak I; Virology Unit, Institute of Microbiology and Parasitology, Veterinary Faculty, University of Ljubljana, Gerbičeva 60, 1000 Ljubljana, Slovenia. |
| Jazyk: |
angličtina |
| Zdroj: |
Pathogens (Basel, Switzerland) [Pathogens] 2021 Jun 25; Vol. 10 (7). Date of Electronic Publication: 2021 Jun 25. |
| DOI: |
10.3390/pathogens10070808 |
| Abstrakt: |
To determine the presence and the prevalence of four different honeybee viruses (acute bee paralysis virus-ABPV, black queen cell virus-BQCV, chronic bee paralysis virus-CBPV, deformed wing virus-DWV) in wild bumblebees, pooled randomly selected bumblebee samples were collected from twenty-seven different locations in the territory of Croatia. All samples were prepared and examined using the RT-PCR methods for quantification of mentioned honeybee viruses. Determined prevalence (%) of identified positive viruses were in the following decreasing order: BQCV > DWV > ABPV, CBPV. Additionally, direct sequencing of samples positive for BQCV ( n = 24) and DWV ( n = 2) was performed, as well as a test of molecular phylogeny comparison with those available in GenBank. Selected positive field viruses' strains showed 95.7 to 100% (BQCV) and 98.09% (DWV) nucleotide identity with previously detected and deposited honeybee virus strains in the geographic areas in Croatia and neighboring Slovenia. In this article, the first detection of four honeybee viruses with genetic characterization of high diversity strains circulating in wild bumblebees in Croatia is presented. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
