| Autor: |
Valencia-Quintana R; Laboratorio 'Rafael Villalobos-Pietrini' de Toxicología Genómica y Química Ambiental, Facultad de Agrobiología, Universidad Autónoma de Tlaxcala, CA Genética y Ambiente UATLX-CA 223, Red Temática de Toxicología de Plaguicidas, Tlaxcala 90120, Mexico., López-Durán RM; Laboratorio de Biomembranas, División de Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana-Iztapalapa, Ciudad de México 09340, Mexico., Milić M; Mutagenesis Unit, Institute for Medical Research and Occupational Health, Ksaverska Cesta 2, 10000 Zagreb, Croatia., Bonassi S; Department of Human Sciences and Quality of Life Promotion, San Rafaele University, 00166 Rome, Italy.; Unit of Clinical and Molecular Epidemiology, IRCCS San Rafaele Pisana, 00166 Rome, Italy., Ochoa-Ocaña MA; Unidad Académica de Estudios Regionales, Coordinación de Humanidades, UNAM, Jiquilpan 59510, Mexico., Uriostegui-Acosta MO; Escuela Superior de Ciencias Naturales, Universidad Autónoma de Guerrero Chilpancingo 39105, Mexico., Pérez-Flores GA; Laboratorio 'Rafael Villalobos-Pietrini' de Toxicología Genómica y Química Ambiental, Facultad de Agrobiología, Universidad Autónoma de Tlaxcala, CA Genética y Ambiente UATLX-CA 223, Red Temática de Toxicología de Plaguicidas, Tlaxcala 90120, Mexico., Gómez-Olivares JL; Laboratorio de Biomembranas, División de Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana-Iztapalapa, Ciudad de México 09340, Mexico., Sánchez-Alarcón J; Laboratorio 'Rafael Villalobos-Pietrini' de Toxicología Genómica y Química Ambiental, Facultad de Agrobiología, Universidad Autónoma de Tlaxcala, CA Genética y Ambiente UATLX-CA 223, Red Temática de Toxicología de Plaguicidas, Tlaxcala 90120, Mexico. |
| Abstrakt: |
Pesticides have been considered as potential chemical mutagens; however, little is known about toxic and genotoxic effects during pesticide application in Zamora-Jacona, Michoacan State in Mexico. This study sought to determine DNA damage and cholinesterase activities inhibitions in 54 agricultural workers exposed to complex mixtures of pesticides vs. control group (26 individuals) using Comet assay in peripheral whole blood, micronucleus (MN) test in oral mucosa cells, Cytokinesis-blocked MN assay in lymphocytes (L-CBMNcyt) and measuring AChE and BChE activities in whole blood and plasma samples, respectively. Exposed subjects demonstrated significantly elevated levels of primary (Comet assay: tail intensity, tail length, tail moment, Olive tail moment) and permanent DNA damage (MN assay: in blood/buccal cells; frequencies of nuclear buds, binucleated cells, cells with condensed chromatin, karyorrhexis, pyknosis, and karyolysis). However, inhibition of cholinesterase activities (AChE and BChE) was not observed in the workers. Confounding factors including sex, age, BMI, working exposure period, protection level, smoking habit (cigarettes per day units), alcohol consumption (weekly), medication, were considered in the analysis. These combined techniques demonstrated usefulness in the health hazards risks pesticide exposure assessment and suggested the need for periodic monitoring together with the education and the training of occupational workers for the safe application of potentially harmful pesticides. |
