Previous estradiol treatment during midlife maintains transcriptional regulation of memory-related proteins by ERα in the hippocampus in a rat model of menopause.

Autor: Baumgartner NE; Brain Institute, Tulane University, New Orleans, LA; Neuroscience Program, Tulane University, New Orleans, LA. Electronic address: nbaumgar@tulane.edu., Black KL; Brain Institute, Tulane University, New Orleans, LA; Neuroscience Program, Tulane University, New Orleans, LA., McQuillen SM; Brain Institute, Tulane University, New Orleans, LA; Neuroscience Program, Tulane University, New Orleans, LA., Daniel JM; Brain Institute, Tulane University, New Orleans, LA; Neuroscience Program, Tulane University, New Orleans, LA; Psychology Department, Tulane University, New Orleans, LA.
Jazyk: angličtina
Zdroj: Neurobiology of aging [Neurobiol Aging] 2021 Sep; Vol. 105, pp. 365-373. Date of Electronic Publication: 2021 Jun 05.
DOI: 10.1016/j.neurobiolaging.2021.05.022
Abstrakt: Previous midlife estradiol treatment, like continuous treatment, improves memory and results in lasting increases in hippocampal levels of estrogen receptor (ER) α and ER-dependent transcription in ovariectomized rodents. We hypothesized that previous and continuous midlife estradiol act to specifically increase levels of nuclear ERα, resulting in transcriptional regulation of proteins that mediate estrogen effects on memory. Ovariectomized middle-aged rats received estradiol or vehicle capsule implants. After 40 days, rats initially receiving vehicle received another vehicle capsule (ovariectomized controls). Rats initially receiving estradiol received either another estradiol (continuous estradiol) or a vehicle (previous estradiol) capsule. One month later, hippocampi were dissected and processed. Continuous and previous estradiol increased levels of nuclear, but not membrane or cytosolic ERα and had no effect on Esr1. Continuous and previous estradiol impacted gene expression and/or protein levels of mediators of estrogenic action on memory including ChAT, BDNF, and PSD-95. Findings demonstrate a long-lasting role for hippocampal ERα as a transcriptional regulator of memory following termination of previous estradiol treatment in a rat model of menopause.
Competing Interests: Disclosure statement None.
(Copyright © 2021. Published by Elsevier Inc.)
Databáze: MEDLINE
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