Electronic signals are electrogenetically relayed to control cell growth and co-culture composition.

Autor:	Stephens K; Fischell Department of Bioengineering, University of Maryland, College Park, USA.; Institute for Bioscience and Biotechnology Research, University of Maryland, College Park, USA.; Robert E. Fischell Institute for Biomedical Devices, University of Maryland, College Park, USA., Zakaria FR; Fischell Department of Bioengineering, University of Maryland, College Park, USA.; Institute for Bioscience and Biotechnology Research, University of Maryland, College Park, USA.; Robert E. Fischell Institute for Biomedical Devices, University of Maryland, College Park, USA., VanArsdale E; Fischell Department of Bioengineering, University of Maryland, College Park, USA.; Institute for Bioscience and Biotechnology Research, University of Maryland, College Park, USA.; Robert E. Fischell Institute for Biomedical Devices, University of Maryland, College Park, USA., Payne GF; Institute for Bioscience and Biotechnology Research, University of Maryland, College Park, USA.; Robert E. Fischell Institute for Biomedical Devices, University of Maryland, College Park, USA., Bentley WE; Fischell Department of Bioengineering, University of Maryland, College Park, USA.; Institute for Bioscience and Biotechnology Research, University of Maryland, College Park, USA.; Robert E. Fischell Institute for Biomedical Devices, University of Maryland, College Park, USA.
Jazyk:	angličtina
Zdroj:	Metabolic engineering communications [Metab Eng Commun] 2021 Jun 13; Vol. 13, pp. e00176. Date of Electronic Publication: 2021 Jun 13 (Print Publication: 2021).
DOI:	10.1016/j.mec.2021.e00176
Abstrakt:	There is much to be gained by enabling electronic interrogation and control of biological function. While the benefits of bioelectronics that rely on potential-driven ionic flows are well known (electrocardiograms, defibrillators, neural prostheses, etc) there are relatively few advances targeting nonionic molecular networks, including genetic circuits. Redox activities combine connectivity to electronics with the potential for specific genetic control in cells. Here, electrode-generated hydrogen peroxide is used to actuate an electrogenetic "relay" cell population, which interprets the redox cue and synthesizes a bacterial signaling molecule (quorum sensing autoinducer AI-1) that, in turn, signals increased growth rate in a second population. The dramatically increased growth rate of the second population is enabled by expression of a phosphotransferase system protein, HPr, which is important for glucose transport. The potential to electronically modulate cell growth via direct genetic control will enable new opportunities in the treatment of disease and manufacture of biological therapeutics and other molecules. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2021 The Authors. Published by Elsevier B.V. on behalf of International Metabolic Engineering Society.)
Databáze:	MEDLINE
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